MARCKS Antibody

Western Blot: MARCKS Antibody [NBP3-05540] - Western blot analysis of different cell lines lysates using MARCKS, at a dilution of 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with mouse mAb to GAPDH at a dilution of 1:5,000 in red, which reveals a single band at 37kDa in all preparations.

Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP3-05540] - Immunofluorescent analysis of HeLa cells stained with MARCKS Antibody at a dilution of 1:5,000 in red, and costained with mouse mAb to beta-tubulin at a dilution of 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The MARCKS antibody binds to MARCKS protein expressed in the plasma membrane and cytoplasm, while the beta-tubulin antibody stains cytoplasmic microtubules.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

MARCKS Antibody Summary

• Description: This antibody is supplied as a concentrated total IgY preparation derived from egg yolk. Production involves an initial organic extraction to remove lipids and lipoproteins, followed by salt fractionation of the aqueous phase to precipitate and enrich the IgY fraction. The material is then extensively dialyzed against PBS, and preservative is added during final formulation.
  
  Please note that the exact concentration of target-specific IgY cannot be determined, as the final preparation contains both antigen-specific IgY and non-immune IgY.
• Immunogen: Full length recombinant human MARCKS expressed in and purified from E. coli.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: MARCKS
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Western Blot 1:5000-1:10000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.
• Preservative: 0.035% Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for MARCKS Antibody

• 80K-L protein
• 80K-L
• FLJ14368
• MACS
• MACSmyristoylated alanine-rich C-kinase substrate
• MARCKS
• MRACKS
• myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
• myristoylated alanine-rich protein kinase C substrate
• phosphomyristin
• PKCSLFLJ90045
• PRKCSL
• Protein kinase C substrate, 80 kDa protein, light chain

Background

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARCKS