Western Blot: MARCKS Antibody [NB110-58875] - Analysis of cell line lysates probed simultaneously with rabbit pAb to MARCKS, dilution 1:1000, in green and mouse mAb to GAPDH, dilution 1:5000 in red:  protein standard ...read more
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NB110-58875] - Analysis of HeLa cells stained with rabbit pAb to MARCKS, NB110-58875, dilution 1:1,000 in red, and costained with mouse mAb to Beta-tubulin, ...read more
This MARCKS antibody is useful for Immunocytochemistry/Immunofluorescence, ABC or other enzyme linked immunocytochemical procedures and Western blotting.
80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NB110-58875 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Supplied as serum
5mM Sodium Azide
Alternate Names for MARCKS Antibody
MACSmyristoylated alanine-rich C-kinase substrate
myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
myristoylated alanine-rich protein kinase C substrate
Protein kinase C substrate, 80 kDa protein, light chain
Actin binding protein MARCKS (myristoylated alanine-rich C kinase substrate) is a major substrate of PKC, and alterations in PKC-MARCKS-actin axis are critical to several biological processes such as motility, proliferation, invasion, adhesion, cell spreading phagocytosis, secretion and membrane trafficking. Intracellular MARCKS is normally attached to cytoplasmic face of plasma membrane and when phosphorylated by activated PKC, MARCKS translocates from plasma membrane to cytoplasm. MARCKS regulates cell movement by controlling actin cytoskeleton homeostasis and filopodium/lamellipodium formation. MARCKS binds to F-actin, but not to monomeric G-actin, independently of its phosphorylation status and when unphosphorylated, it induces cell migration. MAPK8 can phosphorylate MARCKS which leads to induction of actin bundles formation/stabilization followed by reduction in actin plasticity and restricted cell movement including neuronal/cancer cell migration. MARCKS has also been proposed to be involved in coupling the PKC and calmodulin signaling systems. MARCKS is implicated in the pathogenesis of various malignancies such as cancer of pituitary, thyroid, breast, colon, and small intestine as well as melanoma, glioblastoma multiforme cancer and cholangiocarcinoma.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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