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MARCKS Antibody

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Western Blot: MARCKS Antibody [NB110-58875] - Analysis of cell line lysates probed simultaneously with rabbit pAb to MARCKS, dilution 1:1000, in green and mouse mAb to GAPDH, dilution 1:5000 in red: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6 cells. The strong band at ~80kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells [5], but no reactivity is seen on rodent cells, [6] and [7]. GAPDH antibody used as a loading control revealing a single band at ~37kDa in all preparations.
Western Blot: MARCKS Antibody [NB110-58875] - Analysis of cell line lysates probed simultaneously with rabbit pAb to MARCKS, dilution 1:1000, in green and mouse mAb to GAPDH, dilution 1:5000 in red: [1] protein standard ...read more
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NB110-58875] - Analysis of HeLa cells stained with rabbit pAb to MARCKS, NB110-58875, dilution 1:1,000 in red, and costained with mouse mAb to Beta-tubulin, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MARCKS Antibody Summary

Immunogen
Full length recombinant rat MARCKS expressed in and purified from E. coli. [UniProt# P30009]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MARCKS
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:500-1:5000
  • Immunohistochemistry 1:500-1:1000
Application Notes
This MARCKS antibody is useful for Immunocytochemistry/Immunofluorescence, ABC or other enzyme linked immunocytochemical procedures and Western blotting.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB110-58875 in the following applications:

Reactivity Notes

Not reactive to rodent.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as serum
Preservative
5mM Sodium Azide
Purity
Unpurified

Alternate Names for MARCKS Antibody

  • 80K-L protein
  • 80K-L
  • FLJ14368
  • MACS
  • MACSmyristoylated alanine-rich C-kinase substrate
  • MARCKS
  • MRACKS
  • myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
  • myristoylated alanine-rich protein kinase C substrate
  • phosphomyristin
  • PKCSLFLJ90045
  • PRKCSL
  • Protein kinase C substrate, 80 kDa protein, light chain

Background

Actin binding protein MARCKS (myristoylated alanine-rich C kinase substrate) is a major substrate of PKC, and alterations in PKC-MARCKS-actin axis are critical to several biological processes such as motility, proliferation, invasion, adhesion, cell spreading phagocytosis, secretion and membrane trafficking. Intracellular MARCKS is normally attached to cytoplasmic face of plasma membrane and when phosphorylated by activated PKC, MARCKS translocates from plasma membrane to cytoplasm. MARCKS regulates cell movement by controlling actin cytoskeleton homeostasis and filopodium/lamellipodium formation. MARCKS binds to F-actin, but not to monomeric G-actin, independently of its phosphorylation status and when unphosphorylated, it induces cell migration. MAPK8 can phosphorylate MARCKS which leads to induction of actin bundles formation/stabilization followed by reduction in actin plasticity and restricted cell movement including neuronal/cancer cell migration. MARCKS has also been proposed to be involved in coupling the PKC and calmodulin signaling systems. MARCKS is implicated in the pathogenesis of various malignancies such as cancer of pituitary, thyroid, breast, colon, and small intestine as well as melanoma, glioblastoma multiforme cancer and cholangiocarcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MARCKS Antibody (NB110-58875)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


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Bioinformatics

Gene Symbol MARCKS
Entrez