MARCKS Antibody (5F9) [Alexa Fluor™ Plus 488]

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC
• Clone: 5F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

MARCKS Antibody (5F9) [Alexa Fluor™ Plus 488] Summary

• Immunogen: Full length human MARCKS expressed in and purified from E. coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARCKS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MARCKS Antibody (5F9) [Alexa Fluor™ Plus 488]

• 80K-L protein
• 80K-L
• FLJ14368
• MACS
• MACSmyristoylated alanine-rich C-kinase substrate
• MARCKS
• MRACKS
• myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
• myristoylated alanine-rich protein kinase C substrate
• phosphomyristin
• PKCSLFLJ90045
• PRKCSL
• Protein kinase C substrate, 80 kDa protein, light chain

Background

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARCKS