MARCH8 Antibody (710424)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, L1.2 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MARCH8 Monoclonal Antibody (Catalog # MAB6969) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MARCH8 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MARCH8 was detected in immersion fixed immature human dendritic cells using Mouse Anti-Human/Mouse/Rat MARCH8 Monoclonal Antibody (Catalog # MAB6969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Product Discontinued

MARCH8 Antibody (710424) Summary

• Immunogen: E. coli-derived recombinant human MARCH8
  Gln218-Val291
  Accession # Q5T0T0
• Specificity: Detects human MARCH8 in direct ELISAs. In Western blots, no cross-reactivity with recombinant human (rh) MARCH-1 or rhGRAIL is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARCHF8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MARCH8 Antibody (710424)

• Cellular modulator of immune recognition
• c-MIR
• EC 6.3.2
• EC 6.3.2.-
• MARCH8
• MARCH-VIII
• MARCH-VIIIcellular modulator of immune recognition
• membrane-associated ring finger (C3HC4) 8
• Membrane-associated RING finger protein 8
• Membrane-associated RING-CH protein VIII
• MIR
• RING finger protein 178
• RNF178

Background

MARCH8 (membrane-associated RING-CH VIII), also called MIR (modulator of immune recognition), or RNF178 (ring finger 178), is a 33 kDa two-pass transmembrane protein of lysosomal and early endosomal membranes in the MARCH family. MARCH proteins are E3 ubiquitin ligases that promote endocytosis and degradation of their target proteins. MARCH8 ubiquitinates B7-2/CD86 and probably Fas, Tfr and MHC class II proteins such as HLA-A2.1. Within the cytoplasmic C-terminal region used as an immunogen, human MARCH8 shares 87% amino acid identity with mouse and rat MARCH8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARCHF8
• Uniprot:
  • Q5T0T0()