Recombinant Human MAPKAPK2 Protein

Product Discontinued

Recombinant Human MAPKAPK2 Protein Summary

• Description: Recombinant protein for Human MAPKAPK2
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(P49137)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: MAPKAPK2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 254 nmol phosphate incorporated into MAPKAP kinase 2 substrate peptide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP and total of 0.83 uCi/ul P-32.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MAPKAPK2 Protein

• EC 2.7.11
• EC 2.7.11.1
• MAP kinase-activated protein kinase 2
• MAPK-activated protein kinase 2
• MAPKAP kinase 2
• MAPKAPK2
• MAPKAPK-2
• mitogen-activated protein kinase-activated protein kinase 2
• MK2
• Rps6kc1

Background

MAPKAP kinase2 is a Ser/Thr protein kinase, which is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAPK2