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MAP7 Antibody

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Western Blot: MAP7 Antibody [H00009053-B01P] - Analysis of MAP7 expression in transfected 293T cell line by MAP7 polyclonal antibody. Lane 1: MAP7 transfected lysate(84.10 KDa). Lane 2: Non-transfected lysate.
Western Blot: MAP7 Antibody [H00009053-B01P] - Analysis of MAP7 expression in transfected 293T cell line by MAP7 polyclonal antibody. Lane 1: MAP7 transfected lysate(84.10 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MAP7 Antibody [H00009053-B01P] - Analysis of purified antibody to MAP7 on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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MAP7 Antibody Summary

Immunogen
MAP7 (NP_003971.1, 1 a.a. - 749 a.a.) full-length human protein. MAELGAGGDGHRGGDGAVRSETAPDSYKVQDKKNASSRPASAISGQNNNHSGNKPDPPPVLRVDDRQRLARERREEREKQLAAREIVWLEREERARQHYEKHLEERKKRLEEQRQKEERRRAAVEEKRRQRLEEDKERHEAVVRRTMERSQKPKQKHNRWSWGGSLHGSPSIHSADPDRRSVSTMNLSKYVDPVISKRLSSSSATLLNSPDRARRLQLSPWESSVVNRLLTPTHSFLARSKSTAALSGEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPEGSSRRRIIHGTASYKKERERENVLFLTSGTRRAVSPSNPKARQPARSRLWLPSKSLPHLPGTPRPTSSLPPGSVKAAPAQVRPPSPGNIRPVKREVKVEPEKKDPEKEPQKVANEPSLKGRAPLVKVEEATVEERTPAEPEVGPAAPAMAPAPASAPAPASAPAPAPVPTPAMVSAPSSTVNASASVKTSAGTTDPEEATRLLAEKRRLAREQREKEERERREQEELERQKREELAQRVAEERTTRREEESRRLEAEQAREKEEQLQRQAEERALREREEAERAQRQKEEEARVREEAERVRQEREKHFQREEQERLERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAKGALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQNENFEEIINLPIGSKPSRLDVTNSESPEIPLNPILAFDDEGTLGPLPQVDGVQTQQTAEVI
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
MAP7
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
It has been used for WB and Functional.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAP7 Antibody

  • E-MAP-115dJ325F22.2 (microtubule-associated protein 7 (EMAP115, E-MAP-115))
  • ensconsin
  • Epithelial microtubule-associated protein of 115 kDa
  • MAP-7
  • microtubule-associated protein 7EMAP115

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.
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Western Blot: Kinesin 5B Antibody [NB100-1059] - (0.03ug/ml) staining of lysates of cell line HeLa (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Kinesin 5B Antibody [NB100-1059] - (1ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Please note this data was obtained with a previous batch.
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Western Blot: RPS14 Antibody [NBP1-57365] - Jurkat, Antibody Dilution: 1.0 ug/ml RPS14 is supported by BioGPS gene expression data to be expressed in Jurkat.
Western Blot: RPS14 Antibody [NBP1-57365] - Titration: 2.5ug/ml, Positive Control: HepG2 cell lysate.
Western Blot: RPS14 Antibody [NBP1-57365] - Analysis of 721_B cell lysate. Antibody Dilution: 1.0 ug/ml RPS14 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.
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Immunohistochemistry: MTHFR Antibody (5D3) [NBP2-37607] - Immunohistochemical analysis of paraffin-embedded lung cancer using MTHFR mouse mAb with DAB staining.
Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mAb against HEK293 (1) and MTHFR(AA: 339-499)-hIgGFc transfected HEK293 (2) cell lysate.
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Western Blot: PD-ECGF/Thymidine Phosphorylase Antibody [NBP1-84916] - Analysis in human cell line CAPAN-2.
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Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.
Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
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Western Blot: DPYD Antibody (7D4) [H00001806-M01] - DPYD monoclonal antibody (M01), clone 7D4 Analysis of DPYD expression in HeLa.
Immunocytochemistry/Immunofluorescence: DPYD Antibody (7D4) [H00001806-M01] - Analysis of monoclonal antibody to DPYD on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: DPYD Antibody (7D4) [H00001806-M01] - DPYD monoclonal antibody (M01), clone 7D4. Analysis of DPYD expression in Hela S3 NE.
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1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
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Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded mouse brain tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
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Western Blot: MDMX Antibody [NB100-556] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 (lot 2) used for WB at 1 ug/ml (A and B) and used for IP at 6 ug/mg lysate. HdmX/MDM4 was also immunoprecipitated by a previous lot (NB100-556 lot 1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
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Bioinformatics

Gene Symbol MAP7
Uniprot