Lysyl tRNA synthetase Antibody (8G12C1)

Western Blot: Lysyl tRNA synthetase Antibody (8G12C1) [NBP1-47417] - Analysis using KARS mouse mAb against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody (8G12C1) [NBP1-47417] - Analysis of human Tonsil tissues using KARS mouse mAb

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody (8G12C1) [NBP1-47417] - Analysis of human cervical carcinoma, showing cytoplasmic localization with DAB staining using KARS mouse mAb.

Product Discontinued

Lysyl tRNA synthetase Antibody (8G12C1) Summary

• Immunogen: Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KARS1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Lysyl tRNA synthetase Antibody (8G12C1)

• CMTRIB
• EC 6.1.1
• EC 6.1.1.6
• KARS2
• KIAA0070
• KRS
• lysine tRNA ligase
• Lysine--tRNA ligase
• lysRS
• lysyl-tRNA synthetase

Background

lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KARS1
• Entrez:
  • 3735(Human)