Recombinant Mouse LYPLA1 His Protein

SDS-Page: Recombinant Mouse LYPLA1 Protein [NBP1-48592] - 15 % SDS-PAGE (3ug)

• Reactivity: Mu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Mouse LYPLA1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-230 of Mouse LYPLA1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LYPLA1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 26.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Mouse LYPLA1 His Protein

• acyl-protein thioesterase-1
• APT1
• APT-1
• EC 3.1.2
• EC 3.1.2.-
• EC 3.1.2.22
• hAPT1
• LPL1
• LPL1acyl-protein thioesterase 1
• LPL-I
• LYPLA1
• Lysophospholipase 1
• Lysophospholipase I
• lysophospholipase ILYSOPLA
• lysophospholipid-specific lysophospholipase
• LysoPLA I

Background

LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity. Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LYPLA1