LRP-1 Antibody (EPR3724)

Product Discontinued

LRP-1 Antibody (EPR3724) Summary

• Immunogen: A synthetic peptide corresponding to residues in human LRP1 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LRP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:50
  • Western Blot 1:20000-50000
• Application Notes: In Western blot this antibody detects a band at approximately 85/500kDa.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS, 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for LRP-1 Antibody (EPR3724)

• A2MR
• Alpha-2-Macroglobulin Receptor
• APOER
• Apolipoprotein E receptor
• APR
• CD91 antigen
• CD91
• EC 1.1.1.94
• EC 3.4.21.9
• IGFBP3R
• low density lipoprotein receptor-related protein 1
• LRP
• LRP-1
• LRP1A
• prolow-density lipoprotein receptor-related protein 1
• TbetaR-V/LRP-1/IGFBP-3 receptor
• TGFBR5
• type V tgf-beta receptor

Background

Low density lipoprotein receptor-related protein (also known as low density LRP, LRP1, alpha-2-macroglobulin receptor or Apolipoprotein E receptor) is an endocytotic receptor that is involved both in endocytosis and in phagocytosis of apoptotic cells. It is required for early embryonic development, is involved in cellular lipid homeostasis, and may play a role in APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling and neurotransmission. Low density LRP also plays a role in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha-2-macroglobulin), and is involved in the local metabolism of complexes of plasminogen activators and their endogenous ligands. Low density LPR is postulated to be one of the major players in host resistance to HIV. The precursor low density LRP molecule is cleaved post-translationally to form a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515), which remains non-covalently associated with LRP-85. Following cleavage, the intracellular domain (LPRICD) is present in both the cytoplasm and the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Low Density LRP Antibody (NBP1-40726)(4)

Publications using NBP1-40726

• Alabi A, Xia XD, Gu HM et al. Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis Nature communications 2021-03-25 [PMID: 33767172] (WB)
• Chen CH, Shyue SK, Hsu CP, Lee TS. Atypical Antipsychotic Drug Olanzapine Deregulates Hepatic Lipid Metabolism and Aortic Inflammation and Aggravates Atherosclerosis Dis Model Mech 2018-10-10 [PMID: 30355932]
• Yamanaka Y, Faghihi MA, Magistri M et al. Antisense RNA Controls LRP1 Sense Transcript Expression through Interaction with a Chromatin-Associated Protein, HMGB2. Cell Rep. 2015-05-12 [PMID: 25937287]
• Alabi A Insights into Membrane Type-1 Matrix Metalloproteinase cleavage of the Low-density Lipoprotein Receptor Thesis 2021-01-01 (WB)

FAQs for Low Density LRP Antibody (NBP1-40726). (Showing 1 - 2 of 2 FAQs).

1. I have a question about the Low Density LRP antibody with reference number EPR3724. I would like to know the approximate concentration of each vial, although it says in the datasheet that it depends on the vial. I just want to have an approximate information.
   • The concentrations of our latest two lots are 0.1980mg/ml.
2. Does this antibody recognize the extracellular part of LRP so that it can be used on live cells?
   • Low Density LRP Antibody (NBP1-40726) recognizes the cytoplasmic domain of LRP.

Bioinformatics

• Gene Symbol: LRP1
• Entrez:
  • 4035(Human)
  • 16971(Mouse)
  • 299858(Rat)
• Uniprot:
  • Q07954(Human)