LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free

Western Blot: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EDG2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EDG2.

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EDG2.

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free [NBP2-72519] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-EDG2 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human LPAR1(NP_001392) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LPAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 40.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for LPAR1/LPA1/EDG-2 Antibody (OTI1G6) - Azide and BSA Free

• EDG2
• edg-2
• EDG2Lysophosphatidic acid receptor Edg-2
• endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,
• GPCR26
• GPR26
• LPA receptor 1
• LPA1
• LPA-1
• LPA1vzg-1
• LPAR1
• lysophosphatidic acid receptor 1
• Mrec1.3
• rec.1.3
• ventricular zone gene 1
• VZG1
• vzg-1

Background

EDG2 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several proteins not used within 12 hours. Edg2 has been reported in most human tissues, and is especially abundant in brain cortical regions. ESTs have been isolated from bone, brain, breast, connective tissue, embryo, heart/melanocyte/uterus, lung, prostate and uterus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LPAR1/LPA1/EDG-2 Antibody (NBP2-72519)(1)

Publication using NBP2-72519

• Pan H L, Liu B L et al Modulation of Nav1.8 by Lysophosphatidic Acid in the Induction of Bone Cancer Pain Neurosci Bull 2016-09-16 [PMID: 27631681] (WB, IF/IHC, IF/IHC, WB, Rat)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

Bioinformatics

• Gene Symbol: LPAR1