LIR-8/CD85c/LILRB5 Antibody (395239) [Alexa Fluor® 405]

• Applications: WB
• Clone: 395239
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

LIR-8/CD85c/LILRB5 Antibody (395239) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8 isoform 1
  Arg18-His456
  Accession # O75023
• Specificity: Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, 2, 3, 4, 5, rhLIR-6, or rhILT11/LILRA5 is observed. In Western blots, less than 5% cross-reactivity with rhIL
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human LIR-8 (leukocyte immunoglobulin-like receptor 8; also CD85c and LILRB5) is a 567 amino acid (aa) member of the LIR family of receptors. It is a type I transmembrane protein that is expressed on NK cells. LIR-8 has a 435 aa extracellular domain with four C2-type Ig-like domains (aa 24‑458), and a 111 aa cytoplasmic tail that contains two ITIM motifs. Phosphorylation of Tyr in an ITIM motif recruits SHP-1, which is subsequently activated and impacts related signaling pathways. The ligand for, and function of, LIR-8 are unreported. LIR-8 has two potential splice forms. One shows a 53 aa cytoplasmic insert between Arg542 and Ala543. The second shows a deletion of 100 aa between Gly119 and Gly219 that impacts the second C2-type Ig-like domain. There is no rodent ortholog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.