Lipocalin-1 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Lipocalin-1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Lipocalin-1 (R&D Systems, Catalog # 1708-PI)
  His19-Asp176
  Accession # P31025
• Specificity: Detects human Lipocalin-1 in Western blots. In this format, approximately 5% cross‑reactivity with rhLipocalin-2 and rmLipocalin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LCN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-1 Antibody [Biotin]

• LCN1
• lipocalin 1 (tear prealbumin)
• lipocalin 1-like 2
• Lipocalin1
• Lipocalin-1
• MGC71975
• PMFA
• Tear lipocalin
• Tear Prealbumin
• TLC
• TPVon Ebner gland protein
• VEG protein
• VEGP
• VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin)

Background

Lipocalin-1, also known as tear prealbumin or von Ebner’s gland protein (VEGP), is encoded by the LCN1 gene (1 - 3). It is a member of the Lipocalin superfamily that binds many different classes of lipophylic chemicals (4). Lipocalin-1 contains three sequence motifs similar to the cystatins, a superfamily of cysteine protease inhibitors (5). In fact, it has been suggested that Lipocalin-1 is a physiological inhibitor of cysteine proteases and plays a role in the control of inflammatory processes in oral and ocular tissues (5). rhLipocalin-1 corresponds to the mature and secreted protein. It is a weak inhibitor of cysteine proteases such as cathepsin V, which is similar to rhCystatin S.

1. Redl, B. et al. (1992) J. Biol. Chem. 267:20282.
2. Blaker, M. et al. (1993) Biochim. Biophys. Acta 1172:131.
3. Lassagne, H. and A.M. Gachon (1993) Exp. Eye Res. 56:605.
4. Redl, B. et al. (2000) Biochim. Biophys. Acta 1482:241.
5. van’t Hof, W. et al. (1997) J. Biol. Chem. 272:1837.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCN1
• Uniprot:
  • P31025()