LINGO-2 Antibody (382007) [Allophycocyanin]

HEK293 human embryonic kidney cell line transfected with either (A) human LINGO-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LINGO-2 APC-conjugated Monoclonal Antibody (Catalog # FAB36791A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 382007
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

LINGO-2 Antibody (382007) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LINGO-2
  Cys28-Leu542
  Accession # Q7L985
• Specificity: Detects human LINGO-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LINGO-1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LINGO2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LINGO-2 Antibody (382007) [Allophycocyanin]

• LERN3
• LERN3FLJ31810
• leucine rich repeat and Ig domain containing 2
• leucine-rich repeat and immunoglobulin-like domain-containing nogoreceptor-interacting protein 2
• Leucine-rich repeat neuronal protein 3
• Leucine-rich repeat neuronal protein 6C
• LINGO2
• LINGO-2
• LRRN6C
• LRRN6Cleucine rich repeat neuronal 6C

Background

Human LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2), also known as Leucine-Rich Repeat Neuronal 6C (LRRN6C) or LERN3), type I transmembrane protein in the neuronal leucine-rich repeat family. These proteins have a signal peptide, 12 extracellular leucine-rich repeats flanked by N-terminal and C-terminal cysteine-rich sequences, an immunoglobulin-like domain, a transmembrane domain and a short cytoplasmic tail.  An alternate start site may exist at Met148 of the precursor. Human LINGO-2 is a highly conserved, 606 amino acid (aa) protein that shares 99% and 98% aa sequence identity with canine and mouse LINGO-2, respectively. LINGO-2 presumably functions outside the CNS with little involvement by p75/NgR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LINGO2
• Uniprot:
  • Q7L985()