Lgr4/GPR48 Antibody (6G8.B3.G5.C3)

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 6G8.B3.G5.C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Lgr4/GPR48 Antibody (6G8.B3.G5.C3) Summary

• Immunogen: A synthetic peptide corresponding to an internal region of human Lgr4/GPR48 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGR4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This antibody has been tested by ELISA and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to Lgr4/GPR48 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
  
  Suggested applications: IHC

Reactivity Notes

BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Lgr4/GPR48 Antibody (6G8.B3.G5.C3)

• GPR48
• GPR48G protein-coupled receptor 48
• G-protein coupled receptor 48
• leucine-rich repeat containing G protein-coupled receptor 4
• Lgr4

Background

GPR48 is an Orphan-A GPCR with an unknown ligand. GPR48 expression has been documented in human brain, adrenal, heart, kidney, liver, skeletal muscle, ovary, pancreas, placenta, prostate, spleen, stomach, testis, thyroid, and spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR4