LAX1 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

LAX1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human LAX1 isoform 1
  Asn136-Glu398
  Accession # Q8IWV1
• Specificity: Detects human LAX1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAX1 Antibody [Alexa Fluor® 350]

• LAT-like membrane associated protein
• LAX1
• LAXFLJ20340
• Linker for activation of X cells
• lymphocyte transmembrane adapter 1
• lymphocyte transmembrane adaptor 1
• Membrane-associated adapter protein LAX

Background

LAX1 (Linker for activation of X cells) is a 70 kDa member of a small group of T cell transmembrane (TM) adaptor proteins. It is upregulated on both T and B cells after activation, and likely downmodulates both BCR and TCR signaling. Human LAX1 is 398 amino acids (aa) in length. Although its predicted MW is 44 kDa, it runs anomalously at 70 kDa in SDS-PAGE. It is a type III (no signal sequence) TM protein that contains a 37 aa N-terminal extracellular region and a 340 aa cytoplasmic domain (aa 59‑398). There are two potential isoform variants. One has an alternate start site at Met77, while a second shows a 13 aa substitution for aa 1‑29. Over aa 136‑398, human LAX1 is 47% aa identical to mouse LAX1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.