LARG Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

LARG Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human LARG
  Ile154-Leu575
  Accession # Q9NZN5
• Specificity: Detects endogenous human LARG in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LARG Antibody [Alexa Fluor® 405]

• ARHGC
• ARHGEF12
• DKFZp686O2372
• KIAA0382PRO2792
• LARG
• LARGleukemia-associated rho guanine nucleotide exchange factor
• Leukemia-associated RhoGEF
• Rho guanine nucleotide exchange factor (GEF) 12
• rho guanine nucleotide exchange factor 12

Background

LARG (Leukemia-associated RhoGEF, also known as Rho guanine nucleotide exchange factor 12) is a 173 kDa member of the DH‑domain containing RhoGEF family of molecules. It is ubiquitously expressed, and known to interact with semaphorin-activated B class plexins, thereby activating Rho-dependent pathways. Human LARG is 1544 amino acids (aa) in length. It contains one PZD domain that binds to plexin (aa 72‑151), an RGSL region (aa 368‑558), and a RhoGEF domain (aa 788‑975). There are at least two potential splice forms. One shows a deletion of aa 48‑66 with an 8 aa poly-Lys substitution for the C-terminal 960 amino acids. A second shows an insertion of 9 aa between Thr1093 and Asp1094, accompanied by a 6 aa substitution for the C-terminal 131 amino acids. Over aa 154‑575, human LARG is 90% aa identical to mouse LARG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.