Lamin B1 Antibody (4E4) - Azide and BSA Free

Western Blot: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of LMNB1 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of monoclonal antibody to LMNB1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of monoclonal antibody to LMNB1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml

Western Blot: Lamin B1 Antibody (4E4) [H00004001-M03] - LMNB1 monoclonal antibody (M03), clone 4E4. Western Blot analysis of LMNB1 expression in Jurkat ( Cat # L017V1 ).

Western Blot: Lamin B1 Antibody (4E4) [H00004001-M03] - Analysis of LMNB1 expression in Raw 264.7 (Cat # L024V1).

ELISA: Lamin B1 Antibody (4E4) [H00004001-M03] - Lamin B1 Antibody (4E4) [H00004001-M03] - Detection limit for recombinant GST tagged LMNB1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Lamin B1 Antibody (4E4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: LMNB1 (NP_005564.1, 107 a.a. ~ 186 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLAD
• Marker: Nuclear Envelope Marker
• Specificity: LMNB1 - lamin B1 (4E4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LMNB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Lamin B1 Antibody (4E4) - Azide and BSA Free

• ADLD
• Lamin B1
• lamin-B1
• LMN
• LMN2
• LMNB
• LMNB1
• MGC111419

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lamin B1 Antibody (H00004001-M03)(1)

Publication using H00004001-M03

• Khan S, Zuccato J, Ignatchenko V et al. Organelle resolved proteomics reveals new chordoma cell surface markers required for proliferation and association with outcome Research Square 2022-04-13

FAQs for Lamin B1 Antibody (H00004001-M03). (Showing 1 - 1 of 1 FAQ).

1. I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?
   • We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

Bioinformatics

• Gene Symbol: LMNB1
• Entrez:
  • 4001(Human)
• Uniprot:
  • NP_005564(Human)