Kv7.2 Antibody (S26A-23)

Western Blot: Kv7.2 Antibody (S26A-23) [NBP2-12898] - Analysis of Human, hamster HEK and CHO cell lysates showing detection of KCNQ2 protein using Mouse Anti-KCNQ2 Monoclonal Antibody, Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ2 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. KCNQ2 overexpressed.

Immunohistochemistry-Frozen: Kv7.2 Antibody (S26A-23) [NBP2-12898] - Tissue: frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ2 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Immunohistochemistry: Kv7.2 Antibody (S26A-23) [NBP2-12898] - Human hippocampal tissues.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr
• Clone: S26A-23
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Kv7.2 Antibody (S26A-23) Summary

• Immunogen: Fusion protein amino acids 1-59 of human KCNQ2
• Localization: Membrane
• Specificity: Detects approx 95kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNQ2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of KCNQ2 Antibody was sufficient for detection of KCNQ2 in 10 ug of COS-1 cell lysate transiently expressing KCNQ2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Kv7.2 Antibody (S26A-23)

• BFNC
• BFNS1
• EBN
• EBN1
• EIEE7
• ENB1
• HNSPC
• KCNA11
• KCNQ2
• KQT-like 2
• Kv7.2
• KVEBN1
• neuroblastoma-specific potassium channel protein
• Neuroblastoma-specific potassium channel subunit alpha KvLQT2
• potassium voltage-gated channel subfamily KQT member 2
• potassium voltage-gated channel, KQT-like subfamily, member 2
• Voltage-gated potassium channel subunit Kv7.2

Background

1 ug/mL was sufficient for detection of KCNQ2 in 10ug of COS-1 cell lysate transiently expressing KCNQ2 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNQ2