Home » KLHL9 » KLHL9 Lysates » KLHL9 Overexpression Lysate (Denatured)

KLHL9 Overexpression Lysate (Denatured)

 Submit a Review

Images

 
There are currently no images for KLHL9 Lysate (H00055958-T01).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

100% Guarantee on every product

KLHL9 Overexpression Lysate (Denatured) Summary

Description
Plasmid: pCMV-KLHL9 full-length
Gene
KLHL9

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for KLHL9

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-KLHL9 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KLHL9 Overexpression Lysate (Denatured)

  • FLJ13568
  • FLJ21815
  • kelch-like 9 (Drosophila)
  • kelch-like protein 9
  • KIAA1354

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

p19ARF Antibody
NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Immunohistochemistry-Paraffin: p19ARF Antibody [NB200-106] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using p19ARF antibody at 10 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.
(1 Review)
(40 Publications)
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Aurora B Antibody
NB100-294
Immunocytochemistry/Immunofluorescence: Aurora B Antibody [NB100-294] - RPE1 cells stained with Aurora B antibody (green). Image from verified customer review.
Immunohistochemistry: Aurora B Antibody [NB100-294] - Rabbit polyclonal to Aurora B (NB100-294) used to stain SW620 human tumour xenografts (in mouse). The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary.
Chromatin Immunoprecipitation: Aurora B Antibody [NB100-294] - 293T cells stained with Aurora B antibody. Image from verified customer review.
(5 Reviews)
(8 Publications)
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, MiAr
Cullin 3 Antibody
NB100-58788
Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).
Immunohistochemistry: Cullin 3 Antibody [NB100-58788] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
KLHL13 Antibody
(8D1)
NBP1-51623
Western Blot: KLHL13 Antibody (8D1) [NBP1-51623] - Western blot analysis using KLHL13 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: KLHL13 Antibody (8D1) [NBP1-51623] - Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: KLHL13 Antibody (8D1) [NBP1-51623] - Immunohistochemical analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) using KLHL13 mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
ALDH9A1 Antibody
NBP1-89150
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: ALDH9A1 Antibody [NBP1-89150] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Dihydrolipoamide
Dehydrogenase/DLD Ant ...
NBP1-31302
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLD protein stained by DLD antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Paraffin-embedded Gastric CA.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
ILT6/CD85e/LILRA3
Antibody [Unconjugate ...
AF2574
Human peripheral blood monoctyes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human LILRA3/CD85e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2574) or (B) Normal Goat IgG Control(Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Species: Hu
Applications: CyTOF-ready, ICFlow
CDK4 Antibody
NBP1-31308
Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Cdk4 Antibody [NBP1-31308] - Paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
(1 Publication)
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
CDK2AP2 Antibody
NBP1-91776
Western Blot: CDK2AP2 Antibody [NBP1-91776] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: CDK2AP2 Antibody [NBP1-91776] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: CDK2AP2 Antibody [NBP1-91776] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
KLHL22 Antibody
NBP2-17081
Western Blot: KLHL22 Antibody [NBP2-17081] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: KLHL22 Antibody [NBP2-17081] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, IHC, IHC-P
SUB1 Antibody
NBP1-82454
Western Blot: SUB1 Antibody [NBP1-82454] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/Immunofluorescence: SUB1 Antibody [NBP1-82454] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human breast shows strong nuclear positivity in glandular cells.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Recombinant Human
CAC1 Protein
H00143384-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
ADCY10 Antibody
NBP1-81765
Immunohistochemistry-Paraffin: ADCY10 Antibody [NBP1-81765] - Staining of human kidney shows strong positivity in tubules.
Species: Hu
Applications: IHC, IHC-P
KLHL6 Antibody
NBP1-46128
Western Blot: KLHL6 Antibody [NBP1-46128] - Staining of Human (A), Mouse (B) and Rat (C) Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
Kelch-Like Family
Member 21 Antibody
NBP2-30654
Immunohistochemistry-Paraffin: Kelch-Like Family Member 21 Antibody [NBP2-30654] - Staining of human colon shows strong cytoplasmic positivity in a granular pattern in glandular cells.
Species: Hu
Applications: IHC, IHC-P
INCENP Antibody
(3D2)
NBP2-37471
Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Western blot analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: INCENP Antibody (3D2) [NBP2-37471] - Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: INCENP Antibody (3D2) [NBP2-37471] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using INCENP mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
ROBLD3 Antibody
NBP1-71687
Immunocytochemistry/Immunofluorescence: ROBLD3 Antibody [NBP1-71687] - ROBLD3 Antibody was tested at 1:100 in NIH/3T3 cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).
Immunohistochemistry: ROBLD3 Antibody [NBP1-71687] - Analysis of ROBLD3 in human renal cell carcinoma.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
ZNF645 Antibody
NBP2-21037
Western Blot: ZNF645 Antibody [NBP2-21037] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: ZNF645 Antibody [NBP2-21037] - Immunohistochemical analysis of paraffin-embedded GASTRIC CA, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, IHC, IHC-P
S100A9 Antibody
[Unconjugated]
AF2065
Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 20 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
S100A9 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using 10 µg/mL Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counte­rstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(3 Reviews)
(17 Publications)
Species: Mu
Applications: WB, Simple Western, IHC, ICC
KLHL9
Overexpression Lysate
H00055958-T01
KLHL9 Overexpression Lysate (Denatured)
Species: Hu
Applications: WB

Publications for KLHL9 Lysate (H00055958-T01) (0)

There are no publications for KLHL9 Lysate (H00055958-T01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for KLHL9 Lysate (H00055958-T01) (0)

There are no reviews for KLHL9 Lysate (H00055958-T01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KLHL9 Lysate (H00055958-T01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional KLHL9 Products

Bioinformatics Tool for KLHL9 Lysate (H00055958-T01)

Discover related pathways, diseases and genes to KLHL9 Lysate (H00055958-T01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for KLHL9 Lysate (H00055958-T01)

Discover more about diseases related to KLHL9 Lysate (H00055958-T01).
 

Pathways for KLHL9 Lysate (H00055958-T01)

View related products by pathway.

PTMs for KLHL9 Lysate (H00055958-T01)

Learn more about PTMs related to KLHL9 Lysate (H00055958-T01).

Blogs on KLHL9

There are no specific blogs for KLHL9, but you can read our latest blog posts.

Customers Who Bought This Also Bought

KLHL9 Recombinant Protein Antigen
NBP2-54727PEP
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
p19ARF Antibody
NB200-106
Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP2-54727] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.
KLHL9 Antibody
NBP2-54727
Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A9 Antibody [Unconjugated]
AF2065

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KLHL9 Overexpression Lysate (Denatured) and receive a gift card or discount.


Bioinformatics

Gene Symbol KLHL9