Kirrel3/NEPH2 Antibody

Western blot shows lysates of rat embryonic hippocampal neuron. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Kirrel3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4910) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kirrel3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Kirrel3 protein detection in adult human skeletal muscle. A – Western Blot results obtained with anti-human Kirrel3 antibody directed toward intracellular region of Kirrel3 on skeletal muscle lysates obtained from adult human males at baseline, days one and two after completion of a DHR exercise protocol. S = subject B – Western blot results from blocking peptide experiment. The antibody in A was incubated with blocking peptide before being added to membrane containing skeletal muscle lysates obtained from adult human males at baseline and one and two days post completion of a DHR exercise protocol. C – A second commercial antibody directed towards the extracellular domain of Kirrel3 was used on a selection of lysates obtained from adult human male skeletal muscle day1 or 2 post completion of the downhill running exercise bout. Ponceau staining was performed on membranes to demonstrate transfer and equal protein loading. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25488023), licensed under a CC-BY license. Not internally tested by R&D Systems.

Product Discontinued

Kirrel3/NEPH2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kirrel3/NEPH2
  Tyr33-Ala535
  Accession # Q8IZU9
• Specificity: Detects Kirrel3/NEPH2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) Kirrel1 and rhKirrel2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KIRREL3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kirrel3/NEPH2 Antibody

• KIAA1867MGC126824
• kin of IRRE like 3 (Drosophila)
• Kin of irregular chiasm-like protein 3
• kin of IRRE-like protein 3
• KIRRE
• Kirrel3
• KIRREPRO4502
• mKIRRE
• MRD4
• NEPH2
• NEPH2MGC126850
• nephrin-like 2
• Nephrin-like protein 2

Background

Kirrel3 (Kin of IRRE-like protein 3 precursor; also NEPH2) is a 95-120 kDa member of a small family of podocin binding molecules. Kirrel3 is found on podocytes and olfactory sensory neurons and likely plays a role in cellular adhesion. Kirrel3 is 778 amino acids in length. It is a type I transmembrane (TM) glycoprotein that contains a 514 aa extracellular region (aa 22-535) with five C2 type Ig like domains (aa 48-515) plus a 222 aa cytoplasmic tail. Kirrel3 binds itself as well as nephrin, podocin and ZO-1. Kirrel3 is shed by MMPs, generating a soluble urinary fragment that is 25 kDa smaller than the TM form. Two variant isoforms exist. One has an alternate start site at Met34, while the second shows a 35 aa substitution for aa 566-778. Over aa 1-535, human Kirrel3 is 98% aa identical to mouse Kirrel3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kirrel3/NEPH2 Antibody (AF4910)(1)

Publication using AF4910

• Durcan , Peter, Conradie , Johannes, Van deVyver , Mari, Myburgh , Kathryn Identification of novel Kirrel3 gene splice variants in adult human skeletal muscle. BMC Physiol, 2014-12-09;14(1):11. 2014-12-09 [PMID: 25488023] (Western Blot, Human)

Bioinformatics

• Gene Symbol: KIRREL3
• Uniprot:
  • Q8IZU9(Human)