Kir2.2 Antibody (S124B-38) - BSA Free

Western Blot: Kir2.2 Antibody (S124B-38) [NBP2-12901] - analysis of Human Cell lysates showing detection of Kir2.2 Potassium Channel protein using Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody, Clone S124B-38 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Product Discontinued

Kir2.2 Antibody (S124B-38) - BSA Free Summary

• Immunogen: Fusion protein amino acids 362-427 of rat Kir2.2
• Localization: Membrane
• Specificity: Detects approx 45kDa. No cross-reactivity against Kir2.2 or Kir2.3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNJ12
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Kir2.2 Antibody was sufficient for detection of Kir2.2 in 30 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Kir2.2 Antibody (S124B-38) - BSA Free

• ATP-sensitive inward rectifier potassium channel 12
• hIRK
• hIRK1
• hkir2.2x
• Inward rectifier K(+) channel Kir2.2
• Inward rectifier K(+) channel Kir2.2v
• inward rectifier K(+) channel Kir2.6
• IRK2
• IRK-2
• IRK2IRK-2
• KCNJ12
• kcnj12x
• KCNJN1
• KCNJN1FLJ14167
• Kir2.2
• Kir2.2v
• Potassium channel, inwardly rectifying subfamily J member 12
• potassium inwardly-rectifying channel, subfamily J, inhibitor 1
• potassium inwardly-rectifying channel, subfamily J, member 12

Background

1 ug/mL was sufficient for detection of Kir2.2 in 30ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNJ12