Kinesin 5A Antibody

Western Blot: Kinesin 5A Antibody [NB120-5628] - Analysis of kinesin 5A on mouse neuronal cell extract.

Western Blot: Kinesin 5A Antibody [NB120-5628] - Analysis of Kinesin 5A on human retinal extract using PA1-642

Product Discontinued

Kinesin 5A Antibody Summary

• Immunogen: Synthetic Peptide: C(1007) G Y E A E D Q A K L F P L H Q E T A A S(1027)
• Localization: Concentrated in the cell body of the neurons, particularly in the perinuclear region.
• Specificity: This is specific for kinesin 5A and does not detect other kinesin isotypes.
• Predicted Species: Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Detects an approx. 110 kDa (predicted molecular weight: 117 kDa) representing kinesin 5A from mouse neuronal cell lysate and human retinal extracts.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kinesin 5A Antibody

• D12S1889
• KIF5A variant protein
• kinesin family member 5A
• kinesin heavy chain isoform 5A
• Kinesin heavy chain neuron-specific 1
• kinesin, heavy chain, neuron-specific
• MY050
• Neuronal kinesin heavy chain
• NKHC1
• NKHCspastic paraplegia 10 (autosomal dominant)
• SPG10

Background

Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kinesin 5A Antibody (NB120-5628)(1)

Publication using NB120-5628

• Taha E, Patil S, Barrera I et al. eEF2/eEF2K Pathway in the Mature Dentate Gyrus Determines Neurogenesis Level and Cognition Curr. Biol. 2020-07-15 [PMID: 32707059]

Bioinformatics

• Gene Symbol: KIF5A
• Entrez:
  • 3798(Human)
  • 16572(Mouse)