Kinesin 5A Antibody

Product Discontinued

Kinesin 5A Antibody Summary

• Immunogen: Peptide with sequence C-QAKLFPLHQETAAS corresponding to C-Terminus according to NP_004975.2.
• Localization: Concentrated in the cell body of the neurons, particularly the perinuclear region.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KIF5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave bands at approx. 140, 50, 35 and 20 kDa bands in human brain lysates at 1 ug. Please note that currently we cannot find an explanation in scientific literature for the multiple bands we observe given the predicted MW of approx. 119 kDa band according to NP_004975.
• Control Peptide:

  Kinesin 5A Peptide (NB100-1058PEP)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Kinesin 5A Antibody

• D12S1889
• KIF5A variant protein
• kinesin family member 5A
• kinesin heavy chain isoform 5A
• Kinesin heavy chain neuron-specific 1
• kinesin, heavy chain, neuron-specific
• MY050
• Neuronal kinesin heavy chain
• NKHC1
• NKHCspastic paraplegia 10 (autosomal dominant)
• SPG10

Background

Kinesin 5A is part of a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kinesin 5A Antibody (NB100-1058)(1)

Publication using NB100-1058

• Reid E, Kloos M, Ashley-Koch A et al. A kinesin heavy chain (KIF5A) mutation in hereditary spastic paraplegia(SPG10). Am J Hum Genet 2002-11-01 [PMID: 12355402]

Bioinformatics

• Gene Symbol: KIF5A
• Entrez:
  • 3798(Human)
• Uniprot:
  • NP_004975.(Human)