Mouse KIF1B - Ready-To-Use ELISA Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.116 ng/mL (example only; lot dependent)

Mouse KIF1B - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.116 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KIF1B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse KIF1B - Ready-To-Use ELISA Kit (Colorimetric)

• CMT2
• CMT2A
• CMT2A1
• HMSNII
• KIAA0591FLJ23699
• KIAA1448
• kinesin family member 1B
• kinesin superfamily protein KIF1B
• kinesin-like protein KIF1B
• KLP
• MGC134844
• NBLST1

Background

The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. KIF1B is a member of the KIF1/Unc104 family of kinesin-like proteins that are involved in the transport of mitochondria or synaptic vesicles in axons. KIF1B is an amino-terminal-type motor protein that is ubiquitously expressed, with the most abundant levels in differentiated nerve cells. The human KIF1B gene maps to chromosome 1p36.2. Defects in axonal transport due to mutations at the KIF1B gene can underlie the human peripheral neuropathy phenotype. The mouse KIF1B gene generates an alternatively spliced transcript, which produces two isoforms, a 7.8 kb mRNA encoding a 130 kDa protein, and a 10 kb mRNA encoding a 204 kDa protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for KIF1B ELISA Kit (NBP3-55653). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KIF1B