KCTD5 Antibody (OTI3C8) - Azide and BSA Free

Western Blot: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD5.

Immunocytochemistry/Immunofluorescence: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KCTD5.

Immunohistochemistry: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RC200180 mouse monoclonal antibody.

Flow Cytometry: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - HEK293T cells transfected with either pCMV6-ENTRY KCTD5.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-KCTD5 mouse monoclonal, and then analyzed by flow cytometry.

Western Blot: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KCTD5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Immunocytochemistry/Immunofluorescence: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - Staining of HeLa cells using anti-KCTD5 mouse monoclonal antibody.

Immunohistochemistry: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RC200180 mouse monoclonal antibody.

Immunohistochemistry: KCTD5 Antibody (OTI3C8) - Azide and BSA Free [NBP2-71955] - Staining of paraffin-embedded pancreas tissue using anti-RC200180 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

KCTD5 Antibody (OTI3C8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse KCTD5 Antibody (OTI3C8) - Azide and BSA Free (NBP2-02414) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human KCTD5 (NP_061865) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCTD5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-400
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200
• Theoretical MW: 26.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for KCTD5 Antibody (OTI3C8) - Azide and BSA Free

• BTB/POZ domain-containing protein KCTD5
• FLJ20040
• potassium channel tetramerisation domain containing 5

Background

Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCTD5