KCNT2 Antibody (S11-33) - BSA Free

Western Blot: KCNT2 Antibody (S11-33) [NBP1-47606] - analysis of Rat brain membrane lysate showing detection of Slo2.1 Potassium Channel protein using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: KCNT2 Antibody (S11-33) [NBP1-47606] - analysis using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive (nuclei and cytoplasm).

Immunohistochemistry: KCNT2 Antibody (S11-33) [NBP1-47606] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.

Immunohistochemistry: KCNT2 Antibody (S11-33) [NBP1-47606] - Tissue: backskin. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. Image courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Product Discontinued

KCNT2 Antibody (S11-33) - BSA Free Summary

• Immunogen: Fusion protein amino acids 564-624 of mouse Slo2.1 (Slick)
• Localization: Cell Membrane
• Specificity: Detects approx 140kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNT2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of Slo2.1 Antibody was sufficient for detection of Slo2.1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KCNT2 Antibody (S11-33) - BSA Free

• KCa4.2
• MGC119610
• MGC119611
• MGC119612
• potassium channel subfamily T member 2
• potassium channel, subfamily T, member 2
• Sequence like an intermediate conductance potassium channel subunit
• SLICKMGC119613
• SLO2.1
• sodium activated potassium channel
• sodium and chloride activated ATP sensitive potassium channel
• Sodium and chloride-activated ATP-sensitive potassium channel Slo2.1
• sodium- and chloride-activated ATP-sensitive potassium channel
• sodium-and chloride-activated ATP-sensitive potassium channel (SLICK)

Background

FUCNTION: Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. Activated by high intracellular sodium and chloride levels. Channel activity is inhibited by ATP and by inhalation anesthetics, such as isoflurane. Inhibited upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1.; Subcellular location: Cell membrane; Multi-pass membrane protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCNT2 Antibody (NBP1-47606)(1)

Publication using NBP1-47606

• Francisco Ma, Wanggou S, Fan Jj et Al. Chloride intracellular channel 1 cooperates with potassium channel EAG2 to promote medulloblastoma growth J. Exp. Med. 2020-05-04 [PMID: 32097463] (IF, Human)

Bioinformatics

• Gene Symbol: KCNT2
• Entrez:
  • 343450(Human)
• Uniprot:
  • Q6UVM3(Human)