KCNH1 Antibody

Product Discontinued

KCNH1 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of rat KCNH1 (within residues 400-450).[Swiss-Prot# Q63472]
• Localization: Membrane; Multi-pass membrane protein.
• Specificity: This antibody does not cross-react with Kv1.2.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This KCNH1 antibody is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Western blot. In WB, the target band can be seen at ~110 kDa with some additional bands between 110-130 kDa position representing differentially glycosylated form of KCNH1/EAG1 (PMID 25280486 and 15964838). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, bovine and other vertebrates.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KCNH1 Antibody

• EAG channel 1
• EAG1
• EAGeag1
• Ether-a-go-go potassium channel 1
• ether-a-go-go, Drosophila, homolog of
• hEAG1
• h-eageag
• Kv10.1
• MGC142269
• potassium channel, voltage-gated, subfamily H, member 1
• potassium voltage-gated channel subfamily H member 1
• potassium voltage-gated channel, subfamily H (eag-related), member 1
• Voltage-gated potassium channel subunit Kv10.1

Background

KCNH1 (potassium voltage-gated channel subfamily H member 1) is a voltage-gated potassium channel with canonical tetrameric structure and six-transmembrane domain topology. KCNH1 gene was originally named ether a go-go (eag) as its mutation in Drosophila melanogaster induced a rhythmic leg-shaking phenotype under ether anesthesia, and hence also referred as aseag1 or Kv10.1. KCNH1 mutant fruit flies shows a high frequency of spontaneous action potentials and increased transmitter release in motor neurons. In mammals, KCNH1 expression is restricted to brain, where its specific neuronal functions are largely unknown. One of the known KCNH1 physiological functions is its promoting role at the onset of myoblast fusion. KCNH1 has been suspected to play important pathophysiological role in carcinogenesis because the human, KCNH1 gene is overexpressed in a broad spectrum of cancers and its inhibition leads to reduction in cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCNH1 Antibody (NBP1-42816)(6)

Publications using NBP1-42816

• Chavez-Lopez MG, Zuniga-Garcia V, Castro-Magdonel BE et al. Eag1 Gene and Protein Expression in Human Retinoblastoma Tumors and its Regulation by pRb in HeLa Cells Genes (Basel) 2020-01-21 [PMID: 31973216] (WB, Human)
• Garcia-Quiroz J, Garcia-Becerra R, Santos-Martinez N et al. In vivo dual targeting of the oncogenic Ether-a-go-go-1 potassium channel by calcitriol and astemizole results in enhanced antineoplastic effects in breast tumors. BMC Cancer. 2014-10-08 [PMID: 25280486] (WB, ICC/IF, Human)
  
  Details:
  KCNH1/EAG1 antibody used for ICC staining of T-47D and MBCDF cell lines - fixed in 96% ethanol, antigen retrieval via autoclaving in EDTA 0.1 M/pH 9.0, blocking with immunodetector peroxidase blocker/ background Sniper reagents, primary used at 1:300 dilution with 2 hour incubation, detection with immuno-Detector Biotin-Link and immuno-Detector HRP label followed by DAB development (Figure 1). Antibody also used for WB on lysates T-47D xenografts from mice (bearing palpable tumors) treated with vehicle, calcitriol, astemizole or the combination of both drugs - two distinct bands of 110 and 130 kDa observed which might represent differential glycosylation patterns of asparagines at positions 388 and 406 of EAG1 [Figure 5A, results supported by Napp et al. J Biol Chem. 2005 280(33):29506-12].
• de Guadalupe Chavez-Lopez M, Hernandez-Gallegos E, Vazquez-Sanchez AY et al. Antiproliferative and proapoptotic effects of astemizole on cervical cancer cells. Int. J. Gynecol. Cancer. 2014-06-01 [PMID: 24819656] (ICC/IF, Human)
• Garcia-Quiroz J, Garcia-Becerra R, Barrera D et al. Astemizole Synergizes Calcitriol Antiproliferative Activity by Inhibiting CYP24A1 and Upregulating VDR: A Novel Approach for Breast Cancer Therapy PLoS One 2012-01-01 [PMID: 22984610] (IF/IHC, Human)
• Wu W, Dong MQ, Wu XG, Sun HY, Tse HF, Lau CP, Li GR. Human ether-a-go-go gene potassium channels are regulated by EGFR tyrosine kinase. Biochim Biophys Acta;1823(2):282-9. 2012-02-01 [PMID: 22061963] (IP, WB, Human)
• Piros ET, Shen L, Huang XY. Purification of an EH domain-binding protein from rat brain that modulates the gating of the rat ether-a-go-go channel. J Biol Chem;274(47):33677-83. 1999-11-19 [PMID: 10559257] (IP, Human)

Bioinformatics

• Gene Symbol: KCNH1
• Entrez:
  • 3756(Human)
  • 16510(Mouse)
  • 65198(Rat)
• Uniprot:
  • Q63472(Rat)