Home » Kappa Light Chain » Kappa Light Chain Antibodies » Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [DyLight 405]

Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [DyLight 405]

 Submit a Review

Images

 
There are currently no images for Kappa Light Chain Antibody (NBP1-72791V).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alkaline Phosphatase
NBP1-72670
Allophycocyanin/Cy7
NBP1-72791APCCY7-0.5ml
Biotin
NBP1-73641
DyLight 488
NBP1-72791G
DyLight 550
NBP1-72791R
DyLight 594
NBP1-72791DL594
DyLight 650
NBP1-72791C
DyLight 680
NBP1-72791FR
DyLight 755
NBP1-72791IR
FITC
NBP1-73633
Janelia Fluor 549
NBP1-72791JF549
Janelia Fluor 646
NBP1-72791JF646
PE/Atto594
NBP1-72791PEATT594-0.5ml
PE/Cy5.5
NBP1-72791PECY55-0.5ml
PE/Cy7
NBP1-72791PECY7-0.5ml
Rhodamine
NBP1-73627
Texas Red
NBP1-73643
Unconjugated
NBP1-72791
100% Guarantee on every product

Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [DyLight 405] Summary

Immunogen
Mouse kappa light chain
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGKC
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot : 1:5000 - 1:50000
  • ELISA : 1:100000
  • Immunohistochemistry : 1:1000 - 1:5000
  • Immunohistochemistry-Frozen : 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin : 1:1000 - 1:5000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [DyLight 405]

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

POMC Antibody
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(10 Publications)
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
IgG3 Antibody
(RM119) [Unconjugated
NBP2-62125
Immunohistochemistry-Paraffin: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody RM119 at a 1:2000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA
Immunohistochemistry: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - Immunohistochemistry of Human Lymphoid Tissue using NBP2-62125.
ELISA: IgG3 Antibody (RM119) [Unconjugated] [NBP2-62125] - ELISA of human immunoglobulins shows NBP2-62125 only reacts to human IgG3. No cross reactivity with other human immunoglobulins, mouse, rat, or goat IgG.
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, S-ELISA
Recombinant Human
IGKV1-5 GST (N-Term) ...
H00028299-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
CCR2 Antibody
(48607) [Unconjugated
MAB150
CCR2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
(32 Publications)
Species: Hu
Applications: Flow, IHC, CyTOF-ready
CXCL9/MIG [HRP]
DCX900
(3 Reviews)
(27 Publications)
Species: Hu
AIF-1/Iba1 Antibody
NB100-1028
Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of Mouse (A) with antibody at 1 ug/mL and Rat (B) Brain lysate with antibody at 2 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-1028] - A20 silencing prevents the anti-inflammatory effects of EA after focal cerebral ischemia reperfusion. Experiments were performed 24 h after reperfusion in the ischemia/reperfusion cortex, n = 5 per group. The activated morphology of astrocytes and microglia was demonstrated using AIF-1/Iba1 immunofluorescence staining (green). Yellow arrows indicate reactive astrocytes with thick cell bodies, and red arrows indicate hypertrophied activated microglia, noted by their stout, dense appearance with shorter and thicker branched projections. Scale bar = 50 um Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0731-3), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-1028] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
(18 Reviews)
(169 Publications)
Species: Hu, Mu, Rt, Ca, Fe
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
Ig Lambda C
Domain/IGLC2 Antibody
AF6525
Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Human Ig lambda C Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6525) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Immunoglobulin lambda constant region at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB
Progesterone
R/NR3C3 Antibody (4E9
H00005241-M08
Western Blot: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of PGR expression in MCF-7 (Cat # L046V1).
Immunocytochemistry/Immunofluorescence: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on MCF-7 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml
(1 Publication)
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Prion protein
Antibody (SC57-05)
NBP2-67569
Western Blot: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of Prion Protein(PrP) on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded rat brain tissue using anti-PrP antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
CD20 Antibody (L26)
NBP2-44743
Western Blot: CD20 Antibody (L26) [NBP2-44743] - Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).
Immunocytochemistry/Immunofluorescence: CD20 Antibody (L26) [NBP2-44743] - Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (L26) [NBP2-44743] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26)
(1 Publication)
Species: Hu, Pm, Pm, Bv(-), Ca(-), Po(-), Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IF
PDLIM5 Antibody
NBP1-84843
Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).
Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human testis shows no positivity in cells in seminiferous ducts.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
SH2D3C Antibody
NBP2-13306
Western Blot: SH2D3C Antibody [NBP2-13306] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: SH2D3C Antibody [NBP2-13306] - Staining in human spleen and pancreas tissues using anti-SH2D3C antibody. Corresponding SH2D3C RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SH2D3C Antibody [NBP2-13306] - Staining of human spleen shows strong cytoplasmic positivity in subset of cells in red pulp.
Species: Hu
Applications: WB, IHC, IHC-P
Choline
Acetyltransferase/ChA
NBP1-30052
Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.
Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Representative images of lumbar spinal cord sections at 130 days immunostained with Choline Acetyltransferase/ChAT. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
(15 Publications)
Species: Hu, Mu, Rt, Ch, Gp, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl, IHC-WhMt
Calpastatin
Antibody (1F7E3D10)
NB120-5582
Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
(1 Publication)
Species: Hu, Rt, Po, Bv
Applications: WB, ICC/IF, IHC, IHC-P
TGF-beta 2
302-B2
1 μg/lane of Recombinant Human TGF-beta 2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
Recombinant Human TGF-beta 2 (Catalog # 302-B2) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced cell proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.025-0.25 ng/mL.
(3 Reviews)
(57 Publications)
Species: Hu
TGF-beta 3
[Unconjugated]
8420-B3/CF
TGF-beta 3 [Unconjugated]
(3 Publications)
Species: Hu
CPA3 Antibody
NBP1-86130
Western Blot: CPA3 Antibody [NBP1-86130] - Analysis in human plasma.
Immunohistochemistry-Paraffin: CPA3 Antibody [NBP1-86130] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in mast cells.
Western Blot: CPA3 Antibody [NBP1-86130] - Analysis in control (vector only transfected HEK293T lysate) and CPA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Species: Hu
Applications: WB, IHC, IHC-P
CDR1 Antibody
NBP2-57758
Immunocytochemistry/Immunofluorescence: CDR1 Antibody [NBP2-57758] - Staining of human cell line BJ shows localization to nucleoplasm, the Golgi apparatus & centrosome. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF

Publications for Kappa Light Chain Antibody (NBP1-72791V) (0)

There are no publications for Kappa Light Chain Antibody (NBP1-72791V).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Kappa Light Chain Antibody (NBP1-72791V) (0)

There are no reviews for Kappa Light Chain Antibody (NBP1-72791V). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Kappa Light Chain Antibody (NBP1-72791V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alkaline Phosphatase NBP1-72670
Allophycocyanin/Cy7 NBP1-72791APCCY7
Biotin NBP1-73641
DyLight 405 NBP1-72791V
DyLight 488 NBP1-72791G
DyLight 550 NBP1-72791R
DyLight 594 NBP1-72791DL594
DyLight 650 NBP1-72791C
DyLight 680 NBP1-72791FR
DyLight 755 NBP1-72791IR
FITC NBP1-73633
Janelia Fluor 549 NBP1-72791JF549
Janelia Fluor 646 NBP1-72791JF646
PE/Atto594 NBP1-72791PEATT594
PE/Cy5.5 NBP1-72791PECY55
PE/Cy7 NBP1-72791PECY7
Rhodamine NBP1-73627
Texas Red NBP1-73643
Learn about Custom Antibody Labeling

Additional Kappa Light Chain Products

Bioinformatics Tool for Kappa Light Chain Antibody (NBP1-72791V)

Discover related pathways, diseases and genes to Kappa Light Chain Antibody (NBP1-72791V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Kappa Light Chain Antibody (NBP1-72791V)

Discover more about diseases related to Kappa Light Chain Antibody (NBP1-72791V).
 

Pathways for Kappa Light Chain Antibody (NBP1-72791V)

View related products by pathway.

Research Areas for Kappa Light Chain Antibody (NBP1-72791V)

Find related products by research area.

Blogs on Kappa Light Chain

There are no specific blogs for Kappa Light Chain, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Western Blot: AIF-1/Iba1 Antibody [NB100-1028] - Staining of Mouse (A) with antibody at 1 ug/mL and Rat (B) Brain lysate with antibody at 2 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
AIF-1/Iba1 Antibody
NB100-1028

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [DyLight 405] and receive a gift card or discount.


Bioinformatics

Gene Symbol IGKC