Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [Janelia Fluor 549]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [Janelia Fluor 549] Summary

Immunogen
Mouse kappa light chain
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGKC
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Rabbit anti-Mouse Kappa Light Chain Secondary Antibody [Janelia Fluor 549]

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Kappa Light Chain Antibody (NBP1-72791JF549) (0)

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Other Available Formats

Alkaline Phosphatase NBP1-72670
Allophycocyanin/Cy7 NBP1-72791APCCY7
Biotin NBP1-73641
DyLight 405 NBP1-72791V
DyLight 488 NBP1-72791G
DyLight 550 NBP1-72791R
DyLight 594 NBP1-72791DL594
DyLight 650 NBP1-72791C
DyLight 680 NBP1-72791FR
DyLight 755 NBP1-72791IR
FITC NBP1-73633
Janelia Fluor 549 NBP1-72791JF549
Janelia Fluor 646 NBP1-72791JF646
PE/Atto594 NBP1-72791PEATT594
PE/Cy5.5 NBP1-72791PECY55
PE/Cy7 NBP1-72791PECY7
Rhodamine NBP1-73627
Texas Red NBP1-73643

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Bioinformatics

Gene Symbol IGKC
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