Kallikrein 8/Neuropsin Antibody (369910)

• Reactivity: Hu
• Applications: IP, B/N
• Clone: 369910
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 8/Neuropsin Antibody (369910) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8/Neuropsin
  Gln29-Gly260 (predicted)
  Accession # O60259
• Specificity: Detects human Kallikrein 8/Neuropsin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) UPA‑1, rhKallikrein 1, 2, 3, 4, 5, 7, 9, 10, 11, 12, 13, 14, 15, rhKallikrein B1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhProc, or rhThrombin is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: KLK8
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 0.7 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 8/Neuropsin Antibody (369910)

• EC 3.4.21
• EC 3.4.21.118
• hK8
• HNP
• kallikrein 8 (neuropsin/ovasin)
• Kallikrein 8
• kallikrein-related peptidase 8
• KLK8 protein type 1
• KLK8 protein type 2
• KLK8
• neuropsin type 1
• neuropsin type 2
• Neuropsin
• NP
• NRPN
• Ovasin
• PRSS19
• PRSS19kallikrein-8
• Serine protease 19
• Serine protease TADG-14
• TADG14neuropsin
• Tumor-associated differentially expressed gene 14 protein

Background

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid (aa) sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (aa 29‑32) and the mature chain (aa 33-260). Isoform 2 is identical to isoform 1, except that a 45 aa segment is inserted in isoform 2 between aa 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8).

1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
2. Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
3. Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
4. Davies, B. et al. (2001) J. Neurosci. 21:6993.
5. Kishi, T. et al. (2003) Cancer Res. 63:2771.
6. Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
7. Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
8. Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 8/Neuropsin Antibody (MAB17191)(1)

Publication using MAB17191

• Bin L, Kim BE, Hall CF Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin. J. Invest. Dermatol., 2011-07-14;131(11):2213-22. 2011-07-14 [PMID: 21753780] (Neutralization, Human)

Bioinformatics

• Gene Symbol: KLK8
• Uniprot:
  • O60259()