Kallikrein 8/Neuropsin Antibody (189004) [Unconjugated]

Kallikrein 8/Neuropsin was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Kallikrein 8/Neuropsin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 189004
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Kallikrein 8/Neuropsin Antibody (189004) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8/Neuropsin
  Gln29-Gly260 (predicted)
  Accession # O60259
• Specificity: Detects human Kallikrein 8/Neuropsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein 3, 5, 11, or rhHGFA is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLK8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 8/Neuropsin Antibody (189004) [Unconjugated]

• EC 3.4.21
• EC 3.4.21.118
• hK8
• HNP
• kallikrein 8 (neuropsin/ovasin)
• Kallikrein 8
• kallikrein-related peptidase 8
• KLK8 protein type 1
• KLK8 protein type 2
• KLK8
• neuropsin type 1
• neuropsin type 2
• Neuropsin
• NP
• NRPN
• Ovasin
• PRSS19
• PRSS19kallikrein-8
• Serine protease 19
• Serine protease TADG-14
• TADG14neuropsin
• Tumor-associated differentially expressed gene 14 protein

Background

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid (aa) sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide
(aa 29‑32) and the mature chain (aa 33-260). Isoform 2 is identical to isoform 1, except that a 45 aa segment is inserted in isoform 2 between aa 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8).

1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
2. Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
3. Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
4. Davies, B. et al. (2001) J. Neurosci. 21:6993.
5. Kishi, T. et al. (2003) Cancer Res. 63:2771.
6. Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
7. Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
8. Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 8/Neuropsin Antibody (MAB1719)(1)

Publication using MAB1719

• D Netanely, S Leibou, R Parikh, N Stern, H Vaknine, R Brenner, S Amar, RH Factor, T Perluk, J Frand, E Nizri, D Hershkovit, V Zemser-Wer, C Levy, R Shamir Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns Oncogene, 2021-02-09;0(0):. 2021-02-09 [PMID: 33564068] (IHC, Human)

Bioinformatics

• Gene Symbol: KLK8
• Uniprot:
  • O60259()