Recombinant Human Kallikrein 6/Neurosin Protein

Product Discontinued

Recombinant Human Kallikrein 6/Neurosin Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-244 of Human KLK6 full-length ORF
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence:MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWVLTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARPAKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCRYTNWIQKTIQAK

• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: KLK6
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
• Theoretical MW: 53.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0.
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Kallikrein 6/Neurosin Protein

• Bssp
• EC 3.4.21
• EC 3.4.21.-
• hK6
• kallikrein 6 (neurosin, zyme)
• Kallikrein 6
• kallikrein-6
• kallikrein-related peptidase 6
• KLK6
• Klk7
• Neurosin
• Protease M
• protease, serine, 18
• PRSS18
• PRSS9
• PRSS9MGC9355
• Serine protease 18
• Serine protease 9
• SP59
• Zyme

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Multiple alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Kallikrein 6/Neurosin Recombinant Protein (H00005653-P01). (Showing 1 - 1 of 1 FAQ).

1. I purchased Human Kallikrein 6 Recombinant Protein (Cat # H00005653-P01) several months ago to use as a standard for an ELISA procedure. The lot number of the material that we received is 1000316. The Technical Data Sheet that accompanied the protein states that This protein is not active and should not be used for experiments requiring activity. My questions: Does this product contain any enzymatic activity? If the protein is not active, how do you assess activity? By enzyme activity assay? If so, can you provide the data indicating no activity?
   • This recombinant protein actually happens to be one that we distribute for a Taiwanese company called Abnova. We do not perform any testing in house. The reason that the datasheet indicates that this protein is not active is because of the way Abnova synthesizes their recombinant proteins. They use a cell free wheat germ system that is unique to them. With our experience this system does not produce the same post translational modifications and folding that proteins would undergo in an endogenous cell thus rendering them inactive.

Bioinformatics

• Gene Symbol: KLK6