BLTP2 Antibody (A0-F3)

Western Blot: K0100 Antibody (A0-F3) [NBP2-66915] - Analysis on recombinant protein using anti-KIAA0100 Mouse mAb.

Immunocytochemistry/Immunofluorescence: K0100 Antibody (A0-F3) [NBP2-66915] - Staining of Hela cells using anti-KIAA0100 Mouse mAb.

Immunohistochemistry-Paraffin: K0100 Antibody (A0-F3) [NBP2-66915] - Analysis of paraffin- embedded human breast carcinoma tissue using anti-KIAA0100 Mouse mAb.

Product Discontinued

BLTP2 Antibody (A0-F3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human Breast cancer-overexpressed gene 1 protein aa 1,947-2,235 / 2,235. (SwissProt: Q14667 Human)
• Localization: secreted
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIAA0100
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for BLTP2 Antibody (A0-F3)

• Antigen MLAA-22
• BCOX
• BCOX1
• breast cancer overexpressed gene 1
• Breast cancer-overexpressed gene 1 protein
• bridge-like lipid transfer protein family member 2
• cancer/testis antigen 101
• CT101
• DKFZp686M0843
• FMP27
• Hob
• hypothetical protein LOC9703
• KIAA0100
• MGC111488
• MGC134981
• protein hobbit homolog
• U937-associated antigen

Background

This gene was initially characterized in human as having high expression levels in breast carcinomas and breast cancer cell lines. This gene also has increased expression in prostrate cancer cells relative to normal prostrate tissues. Expression of this gene is negatively regulated by direct binding of the microRNA miR-195 to its 3' UTR. miR-195 has been shown to modulate the invasiveness of prostrate cancer cells and xenograft metastases by downgrading expression of this gene. In mouse, the protein encoded by this gene was identified as an antigen on acute monocytic leukemia cells. In human, alternative splicing results in multiple transcript variants encoding distinct isoforms; some of these isoforms are predicted to contain an RNA pol II promoter FMP27 protein domain and a Golgi-body-localization APT1 domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIAA0100