JIP2 Antibody (S135-37)

Product Discontinued

JIP2 Antibody (S135-37) Summary

• Immunogen: Fusion protein amino acids 226-421 of mouse JIP-2/IB-2. Rat: 94% identity (127/135 amino acids identical). Human: 86% identity (173/200 amino acids identical) >50% identity with JIP-1.
• Localization: Cytoplasm
• Specificity: Greater then a 50% identity with JIP-1.Detects approx Greater then 100 kDa. Does not cross-react with JIP-1/IB-1.
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK8IP2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of JIP2/IB2 Antibody was sufficient for detection of JIP2/IB2 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It's predicted to detect Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for JIP2 Antibody (S135-37)

• C-Jun-amino-terminal kinase-interacting protein 2
• homologous to mouse JIP-1
• IB-2
• IB2JIP2Mitogen-activated protein kinase 8-interacting protein 2
• islet-brain 2
• islet-brain-2
• JIP-2
• JNK MAP kinase scaffold protein 2
• JNK MAP kinase scaffold protein JIP2
• JNK-interacting protein 2
• mitogen-activated protein kinase 8 interacting protein 2
• PRKM8 interacting protein-like
• PRKM8IPL

Background

c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites, Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members have been identified as JNK2 and JNK3. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK8IP2
• Uniprot:
  • Q9ERE9(Human)