Recombinant Human ISCU Protein

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SDS-Page: Recombinant Human ISCU Protein [NBP1-50926] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human ISCU Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 35-167 of Human ISCU

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MYHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ISCU
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human ISCU Protein

  • 2310020H20Rik
  • HML
  • hnifU
  • iron-sulfur cluster assembly enzyme ISCU, mitochondrial
  • iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog
  • IscU
  • ISU2
  • MGC74517
  • NIFU
  • NifU-like N-terminal domain containing
  • NifU-like N-terminal domain-containing protein
  • NifU-like protein
  • NIFUN

Background

Iron-sulfur cluster assembly enzyme, also known as ISCU, is a member of the nifU family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. Recombinant human ISCU protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ISCU