IRS2 Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human IRS2 Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

IRS2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IRS2
Pro1035-Ile1136
Accession # Q9Y4H2
Specificity
Detects human IRS2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
IRS2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRS2 Antibody [Unconjugated]

  • insulin receptor substrate 2
  • IRS2
  • IRS-2

Background

IRS2 (insulin receptor substrate 2) is a 180-190 kDa substrate intermediate that lies between various cytokine receptors and downstream signaling molecules. It is the primary IRS protein found in hematopoietic cells. Depending upon the receptor, IRS2 undergoes phosphorylation, either via Tyk2 following type I IFN binding, or via JAK1 and 3 following IL-4, -7 and -15 binding. This event allows for its subsequent association with PI-3 kinase. Human IRS2 is 1338 amino acids (aa) in length with a predicted MW of 138 kDa. It contains one PH (pleckstrin homology) domain (aa 16-144), followed by a PTB (phosphotyrosine-binding)domain (aa 194-298) and seven YxxM (Tyr/x/x/Met) motifs (aa 540-1075). IRS2 is phosphorylated on multiple Ser and Tyr residues. There are two potential splice variants. One shows a 16 aa substitution for the C-terminal Glu, while another shows an 11 aa substitution for aa 1314-1338. Over aa 1035-1136, human IRS2 shares 86% aa identity with mouse IRS2. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IRS2
Uniprot