IRS1 Antibody (744353) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 744353
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

IRS1 Antibody (744353) [Alexa Fluor® 532] Summary

• Immunogen: Phosphopeptide containing the human IRS1 Y1179 site
• Specificity: Detects Human Phospho-IRS1 (Y1179) in direct ELISAs and Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRS1 Antibody (744353) [Alexa Fluor® 532]

• HIRS-1
• insulin receptor substrate 1
• IRS1
• IRS-1

Background

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators.  Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2.  Human IRS1 is 1242 amino acids (aa) in length.  It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225).  IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Insulin receptor-mediated phosphorylation of human IRS1 on Y1179, or its murine equivalent, Y1172, mediates IRS-1 interaction with SH2 domains such as that of the Src family kinase, Fyn, or the tyrosine phosphatase, SHP-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.