IRS1 Antibody (614115) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clone: 614115
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

IRS1 Antibody (614115) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human IRS1
  Ala801-Ala1020 (predicted)
  Accession # P35568
• Specificity: Detects human IRS1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) IRS1 (aa 898-1066) and no cross-reactivity with rmIRS1 (aa 13-113), rmIRS3, or recombinant human IRS2 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRS1 Antibody (614115) [Alexa Fluor® 594]

• HIRS-1
• insulin receptor substrate 1
• IRS1
• IRS-1

Background

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators.  Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2.  Human IRS1 is 1242 amino acids (aa) in length.  It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225).  IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment.  Over aa 801-1020, human IRS1 is 88%, 86% and 88% aa identical to dog, mouse and pig IRS1, respectively.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.