IRS1 Antibody (395601) [Unconjugated]

IRS1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human IRS1 Monoclonal Antibody (Catalog # MAB39781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 395601
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IRS1 Antibody (395601) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IRS1
  Ala801-Ala1020 (predicted)
  Accession # P35568
• Specificity: Detects human and mouse IRS1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRS1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRS1 Antibody (395601) [Unconjugated]

• HIRS-1
• insulin receptor substrate 1
• IRS1
• IRS-1

Background

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators.  Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2.  Human IRS1 is 1242 amino acids (aa) in length.  It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225).  IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment.  Over aa 801-1020, human IRS1 is 88%, 86% and 88% aa identical to dog, mouse and pig IRS1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRS1
• Uniprot:
  • P35568()