IRF2 Antibody (JJ088-0)

Western Blot: IRF2 Antibody (JJ088-0) [NBP2-67870] - Western blot analysis of IRF2 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A

Immunocytochemistry/Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870] - Staining IRF2 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: IRF2 Antibody (JJ088-0) [NBP2-67870] - Analysis of IRF2 on human lung lysates using anti-IRF2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870] - Staining IRF2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IRF2 Antibody (JJ088-0) [NBP2-67870] - Staining IRF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: JJ088-0
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IRF2 Antibody (JJ088-0) Summary

• Description: Novus Biologicals Rabbit IRF2 Antibody (JJ088-0) (NBP2-67870) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human IRF2. (SwissProt: P14316 Human; SwissProt: P23906 Mouse)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IRF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IRF2 Antibody (JJ088-0)

• DKFZp686F0244
• interferon regulatory factor 2
• IRF2
• IRF-2

Background

IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF2