Integrin alpha 7 Antibody (3C12) [FITC]

Product Discontinued

Integrin alpha 7 Antibody (3C12) [FITC] Summary

• Immunogen: This hybridoma was established by fusion of mouse myeloma cell SP2/0 with Integrin alpha7 knockout C57/B6 mouse splenocyte immunized with mouse myoblasts.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 25-50 ug/ml
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 29337118).

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 7 Antibody (3C12) [FITC]

• FLJ25220
• integrin alpha 7 chain
• Integrin alpha 7
• integrin alpha-7
• integrin, alpha 7
• ITGA7

Background

ITGA7 encodes integrin alpha chain 7. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 7 undergoes post-translational cleavage within the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that binds to the extracellular matrix protein laminin-1. Alpha 7 beta 1 is the major integrin complex expressed in differentiated muscle cells. Splice variants of alpha 7 that differ in both the extracellular and cytoplasmic domains exist in the mouse; however, to date only a single human transcript type has been isolated: it contains extracellular and cytoplasmic domains corresponding to the mouse X2 and B variants, respectively. A unique extracellular splice variant has been identified in human, although it clearly represents a minor species and its biological significance is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 7 Antibody (NBP1-54412)(3)

Publications using NBP1-54412

• Endo Y, Baldino K, Li B et al. Loss of ARNT in skeletal muscle limits muscle regeneration in aging FASEB J 2020-10-08 [PMID: 33064329] (Mouse)
• Nixon AML, Duque A, Yelle N et al. A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations. Sci Rep 2019-01-29 [PMID: 30696911] (FLOW, Human)
• Ishii K, Sakurai H, Suzuki N et al. Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro Stem Cell Reports 2018-01-10 [PMID: 29337118] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: ITGA7