INPP5A Antibody (3D8)

Immunoprecipitation: INPP5A Antibody (3D8) [H00003632-M05] - Analysis of INPP5A transfected lysate using anti-INPP5A monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with INPP5A rabbit polyclonal antibody.

Sandwich ELISA: INPP5A Antibody (3D8) [H00003632-M05] - Detection limit for recombinant GST tagged INPP5A is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

INPP5A Antibody (3D8) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: INPP5A (NP_005530, 288 a.a. ~ 387 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YFNQEVFRDNNGTALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDRILMSPSAKELVLRVSVCCPSPGHRGMWSAGSGLAQPW
• Specificity: INPP5A - inositol polyphosphate-5-phosphatase, 40kDa
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: INPP5A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. IHC-P usage was reported in scientific literature. Use in Immunohistochemistry reported in scientific literature (PMID: 30359624).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for INPP5A Antibody (3D8)

• 43 kDa inositol polyphosphate 5-phophatase
• CTCL tumor antigen HD-CL-02,5PTASE
• DKFZp434A1721
• EC 3.1.3.56
• inositol polyphosphate-5-phosphatase, 40kD
• inositol polyphosphate-5-phosphatase, 40kDa
• inositol trisphosphate-5-phosphatase, 40kD
• InsP3 5-phosphatase
• MGC116947,5PTase
• MGC116949
• type I inositol-14,5-trisphosphate 5-phophatase
• type I inositol-14,5-trisphosphate 5-phosphatase

Background

The protein encoded by this gene is a membrane-associated type I inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for INPP5A Antibody (H00003632-M05)(2)

Publications using H00003632-M05

• Cumksy HJ, Costello CM, Zhang N et al. The Prognostic Value of Inositol Polyphosphate 5-Phosphatase in Cutaneous Squamous Cell Carcinoma. J. Am. Acad. Dermatol. 2018-10-22 [PMID: 30359624] (IF/IHC, Human)
• Sekulic A, Kim SY, Hostetter G et al. Loss of inositol polyphosphate 5-phosphatase is an early event in development of cutaneous squamous cell carcinoma. Cancer Prev Res (Phila) 2010-10-01 [PMID: 20876729] (IHC-P, Human)

Bioinformatics

• Gene Symbol: INPP5A
• Entrez:
  • 3632(Human)
• OMIM:
  • 600106(Human)
• Uniprot:
  • NP_005530(Human)