Home » ILF3 » ILF3 Antibodies » ILF3 Antibody

ILF3 Antibody

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: ILF3 Antibody [H00003609-B01P] - Analysis of ILF3 expression in human pancreas.
Western Blot: ILF3 Antibody [H00003609-B01P] - Analysis of ILF3 expression in human pancreas.
Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [H00003609-B01P] - Analysis of purified antibody to ILF3 on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: ILF3 Antibody [H00003609-B01P] - Analysis of purified antibody to ILF3 on formalin-fixed paraffin-embedded human small Intestine. (antibody concentration 3 ug/ml)
Western Blot: ILF3 Antibody [H00003609-B01P] - Analysis of ILF3 expression in HeLa.
Western Blot: ILF3 Antibody [H00003609-B01P] - Analysis of ILF3 expression in transfected 293T cell line by ILF3 polyclonal antibody. Lane 1: ILF3 transfected lysate(77.22 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

ILF3 Antibody Summary

Immunogen
ILF3 (NP_004507, 1 a.a. - 702 a.a.) full-length human protein. MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVEAVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANKKKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEVPPPPNLRGRGRGGSIRGRGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSDFFTDCYGYHDFGSS
Specificity
Reacts with interleukin enhancer binding factor 3, 90kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
ILF3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
This antibody is reactive against tissue and transfected lysate in WB, IF, IHC-P and as a detection antibody in ELISA.
Publications
Read Publication using H00003609-B01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ILF3 Antibody

  • Double-stranded RNA-binding protein 76
  • double-stranded RNA-binding protein, 76 kD
  • DRBF
  • DRBP76M-phase phosphoprotein 4
  • dsRNA binding protein NFAR-2/MPP4
  • interleukin enhancer binding factor 3, 90kDa
  • interleukin enhancer-binding factor 3
  • MPHOSPH4interleukin enhancer binding factor 3, 90kD
  • MPP4MMP4
  • NF90CBTF
  • NFAR-1
  • NFAR2
  • NFARNF110
  • NF-AT-90
  • Nuclear factor associated with dsRNA
  • Nuclear factor of activated T-cells 90 kDa
  • nuclear factor of activated T-cells, 90 kD
  • TCP110
  • TCP80
  • Translational control protein 80

Background

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ILF2 Antibody
NBP1-82586
Western Blot: ILF2 Antibody [NBP1-82586] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: ILF2 Antibody [NBP1-82586] - Staining of human cell line U-251 MG shows localization to nucleoplasm.
Western Blot: ILF2 Antibody [NBP1-82586] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ILF2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(4 Reviews)
(349 Publications)
Species: Hu
Applications: BA
PKR Antibody - BSA
Free
NBP1-77266
Western Blot: PKR Antibody [NBP1-77266] - IAV via Protein Expression Profile in Cell Lines. Antibodies PKR and beta-actin. Secondary: Goat anti-rabbit IgG HRP conjugate.
Western Blot: PKR Antibody [NBP1-77266] - WB Validation of PKR in Human Cell Lines. Antibodies: PKR. Secondary: Goat anti-rabbit IgG HRP conjugate.
Immunohistochemistry-Paraffin: PKR Antibody [NBP1-77266] - Staining of mouse lung tissue with antibody at 5 ug/mL.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Neurofascin
Antibody
AF3235
Western blot shows lysates of rat brain tissue, mouse brain tissue, and human brain (cerebellum). PVDF Membrane was probed with 0.1 µg/mL of Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. Specific bands were detected for Neurofascin at approximately 140 and186 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neurofascin was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglion) using Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (yellow; Catalog # NL016) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and Schwann cells (perinodal regions). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
(1 Review)
(9 Publications)
Species: Hu, Mu, Rt
Applications: IHC, WB
PKLR Antibody
[Unconjugated]
AF8519
Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKLR at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu, Rt
Applications: Simple Western, WB

Glucosylceramidase/GBA
H00002629-M01
Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.
ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.
Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY licence.
(1 Review)
(7 Publications)
Species: Hu, Pl
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(16 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Exportin-5 Antibody
NBP1-89606
Immunocytochemistry/Immunofluorescence: Exportin-5 Antibody [NBP1-89606] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Exportin-5 Antibody [NBP1-89606] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Exportin-5 Antibody [NBP1-89606] - Staining of human testis shows high expression.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
LIN7C Antibody
NBP2-14193
Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
Species: Hu
Applications: IHC, IHC-P
RNA Helicase A
Antibody
NB110-40579
Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by Western blot of immunoprecipitates. Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 (lot NB110-40579-2) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody NB110-40579-1. For blotting immunoprecipitated DHX9, NB110-40579 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunocytochemistry/Immunofluorescence: RNA Helicase A Antibody [NB110-40579] - Immunocytochemistry of KYSE-30 cells to evaluate colocalization of RNA-helicase A (NDHII, green) and RCC2 (red). Colocalization signal is detected in nucleolus. ICC/IF image submitted by a verified customer review.
Western Blot: RNA Helicase A Antibody [NB110-40579] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images (ahnRNPL (NBP1-67852), aILF3 (EPR3627), aLARP1 (NBP1-19128), aMATR3 (NB100-1761), aNCL (NB100-1920)). Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1005121), licensed under a CC-BY licence.
(1 Review)
(6 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
PA2G4/EBP1
Antibody (9J9U5)
NBP3-16561
Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunohistochemistry of paraffin-embedded rat testis using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunohistochemistry of paraffin-embedded mouse kidney using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Guanylate kinase
Antibody (OTI4A8)
NBP2-00594
Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - Analysis of Hela cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - Analysis of Jurkat cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Guanylate kinase antibody, and then analyzed by flow cytometry.
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
PALS1/MPP5 Antibody
NB300-952
Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/Immunofluorescence: PALS1/MPP5 Antibody [NB300-952] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear, plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
PRMT1 Antibody
(779)
NBP2-43744
Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744] - PRMT1 Antibody (779) detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. PRMT1 stained by PRMT1 Antibody (779) diluted at 1:200.
Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744] - PRMT1 Antibody (779) detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. PRMT1 stained by PRMT1 Antibody (779) diluted at 1:200.
Western Blot: PRMT1 Antibody (779) [NBP2-43744] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT1 Antibody (779) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-P, WB
ADAR Antibody
(CL0176)
NBP3-05500
Immunocytochemistry/Immunofluorescence: ADAR Antibody (CL0176) [NBP3-05500] - Staining of A549 cells using the Anti-ADAR monoclonal antibody, showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human cerebral cortex shows nuclear positivity.
Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human testis shows nuclear positivity.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ILF3 Antibody
H00003609-B01P
ILF3 Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for ILF3 Antibody (H00003609-B01P)(1)


Reviews for ILF3 Antibody (H00003609-B01P) (0)

There are no reviews for ILF3 Antibody (H00003609-B01P). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ILF3 Antibody (H00003609-B01P) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ILF3 Products

Bioinformatics Tool for ILF3 Antibody (H00003609-B01P)

Discover related pathways, diseases and genes to ILF3 Antibody (H00003609-B01P). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ILF3 Antibody (H00003609-B01P)

Discover more about diseases related to ILF3 Antibody (H00003609-B01P).
 

Pathways for ILF3 Antibody (H00003609-B01P)

View related products by pathway.

PTMs for ILF3 Antibody (H00003609-B01P)

Learn more about PTMs related to ILF3 Antibody (H00003609-B01P).
 

Research Areas for ILF3 Antibody (H00003609-B01P)

Find related products by research area.

Blogs on ILF3

There are no specific blogs for ILF3, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ILF3 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol ILF3
Entrez
Uniprot