ILF3 Antibody

Images

 
Western Blot: ILF3 Antibody [H00003609-B01P] - Analysis of ILF3 expression in human pancreas.
Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [H00003609-B01P] - Analysis of purified antibody to ILF3 on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: ILF3 Antibody [H00003609-B01P] - Analysis of purified antibody to ILF3 on formalin-fixed paraffin-embedded human small Intestine. (antibody concentration 3 ug/ml)
Western Blot: ILF3 Antibody [H00003609-B01P] - Analysis of ILF3 expression in HeLa.
Western Blot: ILF3 Antibody [H00003609-B01P] - Analysis of ILF3 expression in transfected 293T cell line by ILF3 polyclonal antibody. Lane 1: ILF3 transfected lysate(77.22 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ILF3 Antibody Summary

Immunogen
ILF3 (NP_004507, 1 a.a. - 702 a.a.) full-length human protein. MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVEAVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANKKKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEVPPPPNLRGRGRGGSIRGRGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSDFFTDCYGYHDFGSS
Specificity
Reacts with interleukin enhancer binding factor 3, 90kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
ILF3
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
This antibody is reactive against tissue and transfected lysate in WB, IF, IHC-P and as a detection antibody in ELISA.
Publications
Read Publication using H00003609-B01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ILF3 Antibody

  • Double-stranded RNA-binding protein 76
  • double-stranded RNA-binding protein, 76 kD
  • DRBF
  • DRBP76M-phase phosphoprotein 4
  • dsRNA binding protein NFAR-2/MPP4
  • interleukin enhancer binding factor 3, 90kDa
  • interleukin enhancer-binding factor 3
  • MPHOSPH4interleukin enhancer binding factor 3, 90kD
  • MPP4MMP4
  • NF90CBTF
  • NFAR-1
  • NFAR2
  • NFARNF110
  • NF-AT-90
  • Nuclear factor associated with dsRNA
  • Nuclear factor of activated T-cells 90 kDa
  • nuclear factor of activated T-cells, 90 kD
  • TCP110
  • TCP80
  • Translational control protein 80

Background

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ILF3 Antibody (H00003609-B01P)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ILF3
Entrez
Uniprot