Human IL-10 R beta ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1731
    • Availability
      Product Discontinued

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Human IL-10 R beta ELISA Kit (Colorimetric) Summary

Sensitivity
5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL10RB

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
IL10RB (Human) ELISA Kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of human IL10RB.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): animal serum with 0.09% sodium azide aspreservative. For Standard/Sample (serum/plasma) diluent
  2. Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium/urine) diluent
  3. Detection Antibody IL-10 Rbeta (Item F): biotinylated anti-human IL-10Rbeta (each vial is enough to assay half microplate)
  4. HRP-Streptavidin Concentrate (Item G): 300x concentratedHRP-conjugated streptavidin
  5. IL-10 Rbeta Microplate (Item A): 96 wells (12 strips x 8 wells) coated withanti-human IL-10 Rbeta
  6. Standards (Item C): recombinant human IL-10 Rbeta
  7. Stop Solution (Item I): 2 M sulfuric acid
  8. TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine(TMB) in buffered solution
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human IL-10 R beta ELISA Kit (Colorimetric)

  • CDw210b antigen
  • CDw210b
  • CRF2-4IL-10R subunit 2
  • CRFB4
  • CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B
  • D21S58
  • D21S66
  • IBD25
  • IL-10 R beta
  • IL-10 receptor subunit beta
  • IL10R beta
  • IL-10R2
  • IL-10R2IL-10R subunit beta
  • IL10RB
  • IL-10Rb
  • interleukin 10 receptor, beta
  • Interleukin-10 receptor subunit 2
  • interleukin-10 receptor subunit beta
  • member 4

Background

IL10RB, also known as Interleukin-10 receptor subunit beta, is a 325 amino acid protein that is 37 kDa; belongs to the type II cytokine receptor family; cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29; and its co-expression with IL10RA proteins is needed for IL10-induced signal transduction. This protein is currently being studied for research on several diseases and disorders including inflammatory bowel disease 25, early onset, autosomal recessive, hepatitis b virus, inflammatory bowel disease, hepatitis b, interferon, respiratory syncytial virus bronchiolitis, graft versus host disease, ulcerative colitis crohn's disease, anaplastic large cell lymphoma, secondary hyperparathyroidism, mumps, systemic lupus erythematosus, measles, bronchiolitis, rubella, cblc, lupus erythematosus, rheumatoid arthritis, colitis, and tumors. Interactions with IL10RB have been shown to involve IL22, UCN3, IL10RA, IL28A, and IL10 in cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, toxoplasmosis, tuberculosis, antioxidant action of vitamin-C, immune response IL-22 signaling pathway, and molecular mechanisms of cancer pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for IL-10 R beta ELISA Kit (KA1731). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol IL10RB