IgD Antibody (IgD26)

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Immunohistochemistry-Paraffin: IgD Antibody (IgD26) [NBP3-07422] - Formalin-fixed, paraffin-embedded human Lymph Node stained with IgD Mouse Monoclonal Antibody (IGD26).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
IgD26
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

IgD Antibody (IgD26) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08605)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Full-length human IgD protein (Uniprot: P01880)
Localization
Cytoplasmic
Marker
B-Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHD
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for IgD Antibody (IgD26)

  • IgD
  • Igh-5
  • Ighd
  • Immunoglobulin D
  • Immunoglobulin Delta

Background

Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgD Antibody (NBP3-07422) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for IgD Antibody (NBP3-07422)

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Blogs on IgD.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHD
Uniprot