Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric)

ELISA: Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric) [NBP3-06817] - Samples were spiked with high concentrations of Mouse IFN-gamma R1/CD119 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric) [NBP3-06817] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse IFN-gamma R1/CD119 in samples. No significant cross-reactivity or interference between Mouse IFN-gamma R1/CD119 and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.84
• Intra-Assay: 5.36
• Spike Recovery: 87 - 110 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IFNGR1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IFN-gamma R1/CD119 ELISA Kit (Colorimetric)

• AVP, type 2
• CD119 antigen
• CD119
• FLJ45734
• IFN-gamma R1
• IFN-gamma receptor 1
• IFNgammaR1
• IFN-gamma-R1
• IFNGR
• IFNGR1
• IFN-gR1
• immune interferon receptor 1
• interferon gamma receptor 1
• interferon-gamma receptor alpha chain
• type 2

Background

IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IFN-gamma R1/CD119 ELISA Kit (NBP3-06817). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IFNGR1