IFN-gamma R1/CD119 Antibody (BB1E-2)

Flow Cytometry: IFN-gamma R1/CD119 Antibody (BB1E-2) [NB100-64793] - Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD119: FITC.

Product Discontinued

IFN-gamma R1/CD119 Antibody (BB1E-2) Summary

• Immunogen: Fusion protein of the extracellular part of the human interferon-gamma receptor (AA: Ala17-Gly245) to 11 AA of the c-myc proto-oncogene (for affinity purification).
• Specificity: In Western Blotting only one protein band is stained corresponding to a MW of 43,000, which is equal to the MW for the glycosylated extracellular part of the human extra-cellular interferon-gamma receptor (fused to c-myc), and a MW of 27,000 corresponding to the non-glycosylated part (fused to c-myc). The antibody does not neutralise biological activity of interferon gamma.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNGR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 ug/ml
  • Flow Cytometry 1 ug/ml-10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1 ug/ml-10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
• Control:

  IFN-gamma R1/CD119 Overexpression Lysate

Reactivity Notes

Reacts with Human. Cross reacts with Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for IFN-gamma R1/CD119 Antibody (BB1E-2)

• AVP, type 2
• CD119 antigen
• CD119
• FLJ45734
• IFN-gamma R1
• IFN-gamma receptor 1
• IFNgammaR1
• IFN-gamma-R1
• IFNGR
• IFNGR1
• IFN-gR1
• immune interferon receptor 1
• interferon gamma receptor 1
• interferon-gamma receptor alpha chain
• type 2

Background

The IFNG receptor complex is composed of two type I membrane proteins, IFNGR1 (IFNGR alpha) and IFNGR2 (IFNGR beta). Both receptor proteins are members of the type II cytokine receptor family and share 52% overall sequence identity. The IFNG receptor is constitutively expressed in most cell types. Soluble IFNG has been detected in biological fluids. IFNGR1 is required for ligand-binding and signaling. It binds to IFNG with high affinity and is a potent IFNG antagonist. In signal transduction, IFNG induces tyrosine phosphorylation of IFNGR1 leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFNG-induced gene transcription. Human IFNGR1, a 227 amino acid residue protein, has a calculated molecular mass of approximately 25 kDa. As a result of glycosylation, the recombinant IFNGR1 migrates as a 40 to 50 kDa protein in SDS-PAGE. Human and mouse IFNGRI share approximately 52% amino acid sequence identity and each interacts with IFNG in a strictly species-specific manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma R1/CD119 Antibody (NB100-64793)(1)

Publication using NB100-64793

• Gao J, Shi Lz, Zhao H et al. Loss of IFN-gamma Pathway Genes in Tumor Cells as a Mechanism of Resistance to Anti-CTLA-4 Therapy. Cell 2016-09-09 [PMID: 27667683]

Bioinformatics

• Gene Symbol: IFNGR1