IFN-alpha/beta R2 Overexpression Lysate

Western Blot: IFNAR2 Overexpression Lysate (Adult Normal) [NBP2-10131] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFNAR2.

• Reactivity: Hu
• Applications: WB

IFN-alpha/beta R2 Overexpression Lysate Summary

• Description:

  IFN-alpha/beta R2 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC218039
  
  Accession#: NM_207585
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: IFNAR2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 54.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for IFN-alpha/beta R2 Overexpression Lysate

• beta receptor10
• human interferon alpha
• human interferon alpha/beta receptor
• IFNABR
• IFN-alpha binding protein
• IFN-alpha/beta R2
• IFN-alpha/beta receptor 2
• IFN-alpha-REC
• IFNAR2
• IFN-aR2
• IFNARB
• IFNbR2
• IFN-bR2
• IFN-R
• IFN-R-2
• interferon (alpha, beta and omega) receptor 2
• Interferon alpha binding protein
• interferon alpha/beta receptor 2
• interferon-alpha/beta receptor beta chain
• Type I interferon receptor 2

Background

IFNAR2, also known as Interferon alpha/beta receptor 2, has 3 isoforms, an isoform 1 that is 515 amino acid and 58 kDa, an isoform 2 that is 331 amino acid and 37 kDa, and an isoform3 that is 239 amino acid and 27 kDa; expressed in blood cells, lymphoblastoid and fibrosarcoma cell lines, isoform 3 is detected in the urine; is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta, stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Current research is being performed on several diseases and disorders including interferon, hepatitis c, hepatitis b, multiple sclerosis, measles, hepatocellular carcinoma, liver disease, breast cancer, myeloma, carcinoma, melanoma, adenocarcinoma, respiratory syncytial virus bronchiolitis, portal vein thrombosis, dengue hemorrhagic fever, cervical intraepithelial neoplasia, renal cell carcinoma, hemorrhagic fever, hepatitis c, and japanese encephalitis. This protein has been shown to interact with over 50 proteins including USP18, STAT2, GNB2L1, JAK1, and IFNA13 in cytokine-cytokine receptor interaction, osteoclast differentiation, toll-like receptor signaling pathway, Jak-STAT signaling pathway, natural killer cell mediated cytotoxicity, hepatitis C, measles, THC differentiation pathway, regulation of IFNA signaling and influenza A pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IFNAR2