IFN-alpha/beta R2 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

IFN-alpha/beta R2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human IFN-alpha / beta R2 C-Terminus
  Ser351-Met514
  Accession # P48551
• Specificity: Detects human IFN-alpha / beta R2 C-Terminus in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IFN-alpha / beta R2a, rhIFN-alpha / beta R alpha , and recombinant mouse IFN-alpha / beta R2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha/beta R2 Antibody [Alexa Fluor® 647]

• beta receptor10
• human interferon alpha
• human interferon alpha/beta receptor
• IFNABR
• IFN-alpha binding protein
• IFN-alpha/beta R2
• IFN-alpha/beta receptor 2
• IFN-alpha-REC
• IFNAR2
• IFN-aR2
• IFNARB
• IFNbR2
• IFN-bR2
• IFN-R
• IFN-R-2
• interferon (alpha, beta and omega) receptor 2
• Interferon alpha binding protein
• interferon alpha/beta receptor 2
• interferon-alpha/beta receptor beta chain
• Type I interferon receptor 2

Background

IFN-alpha / beta R2 (Interferon alpha/beta receptor 2/IFNAR2; also IFN alpha binding protein) is a 90-102 kDa member of the class II cytokine receptor family of molecules. It is expressed on hematopoietic cells, including T cells, NK cells, B cells and monocytes. It serves as a ligand binding subunit for type I IFN, and generates a functional IFN receptor by complexing with 125 kDa IFNAR1. Multiple type I IFNs signal through this heterodimeric receptor with distinct outcomes. This may be attributable to unique structural conformations created by the Type I IFN:IFNAR1 interaction, or the use of a truncated isoform of IFNAR2. Mature human IFNAR2 is a 489 amino acid (aa) type I transmembrane glycoprotein (aa 27-515). It contains a 217 aa extracellular region (aa 27-243) that contains an Ig-like domain (aa 39-118), a fibronectin type III domain, and a 251 aa cytoplasmic tail. There are two isoform variants. The first is 50-55 kDa in size and contains a 51 aa substitution for aa 281-515. The second is a 40 kDa soluble form that shows a two aa substitution for aa 238-515. IFNAR2 also undergoes proteolysis. Cleavage of the 92 kDa isoform generates a 58 kDa membrane-embedded stub that can be further cleaved into 27-35 kDa fragments that undergo nuclear translocation. Over aa 351-514. human IFNAR2 shares 48% aa identity with mouse IFNAR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P48551(Human)