Recombinant Human IFN-alpha 2 Protein

Product Discontinued

Recombinant Human IFN-alpha 2 Protein Summary

• Description: A recombinant protein with a 16 residue His tag and corresponding to 182 total residues of IFNA2.
  
  Source:E. coli The recombinant protein migrates with an apparent molecular mass of 24 kDa in SDS-PAGE.
• Specificity: NovActiverecombinant human IFN-alpha-2a is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
• Protein/Peptide Type: Recombinant Protein
• Gene: IFNA2

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Functional
  • Western Blot
• Application Notes: This protein is functionally active and can be added to cell culture without the need to remove any Bioactive molecules. Can also be used for Western Blot.
  
  Bioactivity is assayed by measuring the dose dependent cytotoxicity of human IFN-alpha-2a on TF-1 in the presence of IL-4. The ED50 for this batch of NovActiverecombinant human IFN-alpha-2a was determined to be 0.108 ng/mg, corresponding to specific activity of 9.26x10E6 U/mg.

Reactivity Notes

Human Recombinant protein

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered through a 0.2 um filter.PBS, pH 7.2.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute with deionized water.

Alternate Names for Recombinant Human IFN-alpha 2 Protein

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

Interferon alpha 2a (IFN-alpha-2a) belongs to class I interferons. Interferons are produced by the cells of the innate immune system as a part of the defense response to foreign agents such as viruses, parasites and tumor cells. Interferons (class I) bind to a specific cell-surface receptor leading to the transcription and translation of genes coding for proteins having antiviral, antiproliferate and antioncogenic effects, as well as modulating the immune response. IFN-alpha-2a inhibits viral replication within host cells, activates natural killer cells and macrophages and increases antigen presentation to T-lymphocytes. It has been used in the treatment of hepatitis C and various cancers, including leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Interferon alpha 2a Protein (NBC1-21338). (Showing 1 - 1 of 1 FAQ).

1. I would like to now how to correspond ED50 ng/ml to U/ml. IFN-alpha-2b was determined to be 0.16 ng/ml, corresponding to specific activity of how many U/ml?
   • The specific activity of this protein is 9.26x10E6 U/mg, with an ED50 of 0.108 ng/mg.

Bioinformatics

• Gene Symbol: IFNA2